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Lamin B1 Antibody (YA3871)

(Synonyms: LMN; ADLD; LMN2; LMNB; MCPH26)
Customer Review

Based on 1 Customer Validation

Lamin B1 Antibody (YA3871) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LMNB1. It can be used as a loading control antibody.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Lamin B1 Antibody (YA3871) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

Lamin B1 Antibody (YA3871) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LMNB1. It can be used as a loading control antibody.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 66 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 66 kDa
Immunogen

Purified recombinant fragment of human LMNB1 (AA: 413-583) expressed in HEK293-6e cells supernatant.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718853

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    Western blot analysis of extracts from RAW264.7(lane 2(20μg) , HEK293(lane 3(20μg) ,THP-1(lane 4(20μg)and C2C12( lane 5(20μg) using Lamin B1 Antibody (HY-P84174). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    Flow cytometric analysis of 1X106 HeLa cells labeling Lamin B1 Antibody (HY-P84174, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Lamin B1 is a Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively[1][2].

  • Subcellular Localization

    Nucleus lamina

  • Subunit

    Homodimer (PubMed:22265972, PubMed:33706103). Lamin dimers then assemble into dimeric head-to-tail polymers.

  • SwissProt ID

    P20700 

  • Gene ID
    4001 [NCBI]
  • Synonyms

    LMN; ADLD; LMN2; LMNB; MCPH26

Lamin B1 Antibody (YA3871) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20μg) , HEK293(lane 3(20μg) ,THP-1(lane 4(20μg)and C2C12( lane 5(20μg) using Lamin B1 Antibody (HY-P84174). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling Lamin B1 Antibody (HY-P84174, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Lamin B1 Antibody (YA3871) Product Image
    1/16
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Lamin B1 Antibody (YA3871) Product Image
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20μg) , HEK293(lane 3(20μg) ,THP-1(lane 4(20μg)and C2C12( lane 5(20μg) using Lamin B1 Antibody (HY-P84174). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling Lamin B1 Antibody (HY-P84174, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Experimental Validation Results for Lamin B1 Antibody (YA3871)
  • Lamin B1 Antibody (YA3871) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from RAW264.7(lane 2(20μg) , HEK293(lane 3(20μg) ,THP-1(lane 4(20μg)and C2C12( lane 5(20μg) using Lamin B1 Antibody (HY-P84174). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

  • Lamin B1 Antibody (YA3871) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lamin B1 Antibody (YA3871) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lamin B1 Antibody (YA3871) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lamin B1 Antibody (YA3871) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lamin B1 Antibody (YA3871) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lamin B1 Antibody (YA3871) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using Lamin B1 Antibody (HY-P84174, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lamin B1 Antibody (YA3871) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling Lamin B1 Antibody (HY-P84174, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Lamin B1 Antibody (YA3871) Product Image WB
  • Lamin B1 Antibody (YA3871) Product Image IHC-P
  • Lamin B1 Antibody (YA3871) Product Image IHC-P
  • Lamin B1 Antibody (YA3871) Product Image IHC-P
  • Lamin B1 Antibody (YA3871) Product Image IHC-P
  • Lamin B1 Antibody (YA3871) Product Image IHC-P
  • Lamin B1 Antibody (YA3871) Product Image IHC-P
  • Lamin B1 Antibody (YA3871) Product Image FC