IGF2BP3 Antibody (YA3490)(PBS only)

Cat. No.: HY-P83793A: Data Sheet User Guide for Antibodies
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IGF2BP3 Antibody (YA3490) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IGF2BP3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

IGF2BP3 Antibody (YA3490) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IGF2BP3.

Host

Mouse

Molecular Weight

Predicted band size: 64 kDa;

Observed band size: 70-90 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O00425

Gene ID

10643 [NCBI]

Immunogen

Purified recombinant fragment of human IGF2BP3 (AA: 430-579) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability (PubMed:29476152). Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Increases MYC mRNA stability by binding to the coding region instability determinant (CRD) and binding is enhanced by m6A-modification of the CRD (PubMed:29476152). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs

Subcellular Localization：Nucleus; Cytoplasm; Cytoplasm, P-body; Cytoplasm, Stress granule

Expression：
Tissue_specificity:It is expressed (protein level) in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal ovarian follicles and testicular germ cells, developing oocytes, spermatogonia, and semen. It is also expressed (protein level) in cervical adenocarcinoma, testicular cancer, pancreatic cancer, and renal cell carcinoma. It is widely expressed during fetal development at 8 and 14 weeks of gestation. It is also expressed in ovarian, testicular, brain, placenta, pancreatic cancer tissue, and pancreatic cancer cell lines.

Isoforms & Post-Translational Modification：O00425 has 2 isomers: O00425-1: 63705 Da (predicted); O00425-2: 21630 Da (predicted).

Subunit：Can form homooligomers and heterooligomers with IGF2BP1 and IGF2BP3 in an RNA-dependent manner (PubMed:23640942). Interacts with IGF2BP1 (PubMed:17289661). Interacts with ELAVL1, DHX9, HNRNPU, MATR3 and PABPC1 (PubMed:23640942, PubMed:29476152)

Synonyms

KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3

Documentation

User Guide for Antibodies (1077 KB)