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Catalase Antibody (YA6310)

(Synonyms: CAT; Catalase)
Customer Review

Based on 1 Customer Validation

Catalase Antibody (YA6310) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Catalase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG/Kappa

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Catalase Antibody (YA6310)
  • Experimental Validation Results for Catalase Antibody (YA6310)
  • Catalase Antibody (YA6310) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1000 1:1000-5000 1:200-1000 1:5000-20000

Product Details

Description

Catalase Antibody (YA6310) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Catalase.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 60 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG/Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Catalase Antibody (YA6310)
    Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), 293T (lane 3), HT-1080 (lane 4), and SH-SY5Y (lane 5) using Catalase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Catalase Antibody (YA6310)
    Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), 293T (lane 3), HT-1080 (lane 4), SH-SY5Y (lane 5), HepG2 (lane 6) and THP-1 (lane 7) using Catalase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Catalase catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide. Promotes growtH2O2 cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells[1].

  • Subcellular Localization

    Peroxisome matrix

  • Subunit

    Homotetramer (PubMed:10656833, PubMed:10666617, PubMed:21976670). Interacts (via microbody targeting signal) with PEX5, monomeric form interacts with PEX5, leading to its translocation into peroxisomes (PubMed:21976670)

  • SwissProt ID

    P04040

  • Gene ID
    847 [NCBI]
  • Synonyms

    CAT; Catalase

Catalase Antibody (YA6310) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), 293T (lane 3), HT-1080 (lane 4), and SH-SY5Y (lane 5) using Catalase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), 293T (lane 3), HT-1080 (lane 4), SH-SY5Y (lane 5), HepG2 (lane 6) and THP-1 (lane 7) using Catalase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Catalase Antibody (YA6310) Product Image
    1/16
  • Experimental Validation Results for Catalase Antibody (YA6310)
  • Experimental Validation Results for Catalase Antibody (YA6310)
  • Catalase Antibody (YA6310) Product Image
  • Experimental Validation Results for Catalase Antibody (YA6310)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), 293T (lane 3), HT-1080 (lane 4), and SH-SY5Y (lane 5) using Catalase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Catalase Antibody (YA6310)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), 293T (lane 3), HT-1080 (lane 4), SH-SY5Y (lane 5), HepG2 (lane 6) and THP-1 (lane 7) using Catalase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Catalase Antibody (YA6310)
  • Experimental Validation Results for Catalase Antibody (YA6310)
  • Catalase Antibody (YA6310) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), 293T (lane 3), HT-1080 (lane 4), and SH-SY5Y (lane 5) using Catalase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Catalase Antibody (YA6310) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), 293T (lane 3), HT-1080 (lane 4), SH-SY5Y (lane 5), HepG2 (lane 6) and THP-1 (lane 7) using Catalase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Catalase Antibody (YA6310) Product Image WB
  • Catalase Antibody (YA6310) Product Image WB