CDC45 Antibody (YA3158)

Cat. No.: HY-P83413: Data Sheet COA
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User Guide for Antibodies
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CDC45 Antibody (YA3158) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDC45.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CDC45 Antibody (YA3158) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

CDC45 Antibody (YA3158) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDC45.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 66 kDa;

Observed band size: 66 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O75419

Gene ID

8318 [NCBI]

Immunogen

A synthesized peptide derived from human CDC45 aa500-566/566.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), K562 (lane 2, 15 μg), NCI-H1299 (lane 3, 15 μg), and Hela (lane 4, 15 μg) using CDC45 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), MCF-7 (lane 3), 293T (lane 4), HT-1080 (lane 5), A549 (lane 6), and HepG2 (lane 7, Negative), using CDC45 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot^® Turbo^™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

Function：Required for initiation of chromosomal DNA replication. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built

Subcellular Localization：Nucleus; Chromosome

Expression：
Tissue_specificity:Widely expressed, highest levels are found in human testis and thymus and in fetal liver

Isoforms & Post-Translational Modification：O75419 has 3 isomers: O75419-1: 65569 Da (predicted); O75419-2: 60245 Da (predicted); O75419-3: 68768 Da (predicted).

Subunit：Component of the CMG helicase complex, a hexameric ring of related MCM2-7 subunits stabilized by CDC45 and the tetrameric GINS complex (PubMed:32453425, PubMed:34694004, PubMed:34700328). Associated with ORC2. Interacts with HELB (PubMed:25933514)

Database

Entrez Gene: 8318 Human ; 12544 Mouse ; 287961 Rat

SwissProt: O75419 Human ; Q9Z1X9 Mouse ;

OMIM: 617063 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

CDC45L; CDC45L2; PORC-PI-1; CDC45

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

CDC45 Antibody (YA3158) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CDC45 Antibody (YA3158)

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CDC45 Antibody (YA3158) Related Classifications

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