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  4. IRS1 Antibody

IRS1 Antibody

Cat. No.: HY-P81116
COA User Guide for Antibodies

IRS1 Antibody is an unconjugated, approximately 137 kDa, rabbit-derived, anti-IRS1 polyclonal antibody. IRS1 Antibody can be used for: WB, ELISA, IHC-P, IHC-F, IF expriments in human, mouse, rat and predicted: chicken background without labeling.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL $75 In-stock
50 μL $190 In-stock
100 μL $310 In-stock
250 μL   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

IRS1 Antibody is an unconjugated, approximately 137 kDa, rabbit-derived, anti-IRS1 polyclonal antibody. IRS1 Antibody can be used for: WB, ELISA, IHC-P, IHC-F, IF expriments in human, mouse, rat and predicted: chicken background without labeling.

Background

The related gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in The related gene are associated with type II diabetes and susceptibility to insulin resistance.

Gene ID

3667

Synonyms
IRS1_HUMAN; Insulin receptor substrate 1; IRS-1; IRS 1;
Molecular Weight

Predicted band size: 137kDa

Purity

affinity purified

Subcellular Location

IRS1 is predominantly found in the cytoplasm. Nuclear localization may occur in some cell types and under specific stimuli.

Conjugation

Non-conjugated

Modification

Unmodified

Product Categories

Primary Antibody; Rabbit Polyclonal Antibody

Clonality

Polyclonal

Host

Rabbit

Reactivity

Human, Mouse, Rat

Predicted Reactivity

Chicken

Note: The predicted reactivity is for reference only, not as a product performance guarantee.
SwissProt ID

P35568

Dilution Ratio

WB: 1:500-2000;ELISA: 1:5000-10000;IHC-P: 1:100-500;IHC-F: 1:100-500;IF: 1:100-500

  • Western blot analysis of extracts from 293T (lane 1) and 3T3 (lane 2) using IRS1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application

WB, ELISA, IHC-P, IHC-F, ICC/IF

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

KLH conjugated synthetic peptide derived from rat IRS-1: 1101-1200/1242

Documentation

IRS1 Antibody Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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IRS1 Antibody
Cat. No.:
HY-P81116
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