IRS-1 Antibody

Cat. No.: HY-P81116: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

IRS-1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to IRS-1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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IRS-1 Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

IRS-1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to IRS-1.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 132 kDa;

Observed band size: 160-185 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat Predicted Reactivity: Chicken

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P35568

Gene ID

3667 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from rat IRS-1: 1101-1200/1242

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-10000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R (PubMed:7541045, PubMed:33991522, PubMed:38625937). Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism (PubMed:19639489). Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2 (PubMed:11171109, PubMed:8265614). Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade (By similarity). Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation (PubMed:24616100)

Subcellular Localization：Cytoplasm; Nucleus

Subunit：Interacts with UBTF and PIK3CA (By similarity). Interacts with SOCS7 (PubMed:16127460).

RRID

AB_3103066

Database

Entrez Gene: 3667 Human ; 16367 Mouse ; 25467 Rat

SwissProt: P35568 Human ; P35569 Mouse ; P35570 Rat

OMIM: 125853 Human

Synonyms

IRS1_HUMAN; Insulin receptor substrate 1; IRS-1; IRS 1;

Documentation

Select Batch:

Data Sheet (233 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

IRS-1 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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IRS-1 Antibody

IRS-1 Antibody Featured Recommendations:

Product Detail

Background

Documentation

IRS-1 Antibody Related Classifications

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