2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. RICTOR Antibody (YA5903)

RICTOR Antibody (YA5903)

製品番号: HY-P86211
User Guide for Antibodies Technical Support

RICTOR Antibody (YA5903) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RICTOR.

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容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

RICTOR Antibody (YA5903) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RICTOR.
Host

Rabbit
Clonality

Monoclonal
分子量
Predicted band size: 192 kDa;
Observed band size: 192 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse
SwissProt ID
Gene ID
Application &
Dilution Ratio
Application Dilution Ratio
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:200-1:1000
WB
WB: Western Blot
1:2000-1:10000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:200-1:1000
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:5000-1:20000
IP
IP: Immunoprecipitation
1:50-1:200
純度 Protein A Conjugation Non-conjugated
Modification Unmodified Isotype IgG
Appearance

Liquid
Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Component of the mechanistic target of rapamycin complex 2 (mTORC2), which transduces signals from growth factors to pathways involved in proliferation, cytoskeletal organization, lipogenesis and anabolic output (PubMed:15268862, PubMed:15718470, PubMed:19720745, PubMed:19995915, PubMed:21343617, PubMed:33158864, PubMed:35904232, PubMed:35926713). In response to growth factors, mTORC2 phosphorylates and activates AGC protein kinase family members, including AKT (AKT1, AKT2 and AKT3), PKC (PRKCA, PRKCB and PRKCE) and SGK1 (PubMed:19720745, PubMed:19935711, PubMed:19995915). In contrast to mTORC1, mTORC2 is nutrient-insensitive (PubMed:15467718, PubMed:21343617). Within the mTORC2 complex, RICTOR probably acts as a molecular adapter (PubMed:21343617, PubMed:33158864, PubMed:35926713). RICTOR is responsible for the FKBP12-rapamycin-insensitivity of mTORC2 (PubMed:33158864). mTORC2 plays a critical role in AKT1 activation by mediating phosphorylation of different sites depending on the context, such as 'Thr-450', 'Ser-473', 'Ser-477' or 'Thr-479', facilitating the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDPK1/PDK1 which is a prerequisite for full activation (PubMed:15718470, PubMed:19720745, PubMed:19935711, PubMed:35926713). mTORC2 catalyzes the phosphorylation of SGK1 at 'Ser-422' and of PRKCA on 'Ser-657' (By similarity). The mTORC2 complex also phosphorylates various proteins involved in insulin signaling, such as FBXW8 and IGF2BP1 (By similarity). mTORC2 acts upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors (PubMed:15467718). mTORC2 promotes the serum-induced formation of stress-fibers or F-actin (PubMed:15467718)
Subcellular Localization：Cell membrane; Endoplasmic reticulum membrane; Lysosome membrane
Isoforms & Post-Translational Modification：Q6R327 has 3 isomers: Q6R327-1: 192218 Da (predicted); Q6R327-4: 28988 Da (predicted); Q6R327-3: 195011 Da (predicted).
Phosphorylated by MTOR; when part of mTORC2 (PubMed:15467718). Phosphorylated at Thr-1135 by RPS6KB1 downstream of the mTORC1 complex: phosphorylation of RICTOR inhibits mTORC2 signaling by creating a binding site for 14-3-3 proteins (PubMed:19720745, PubMed:19935711, PubMed:19995915). Phosphorylated at Thr-1695 by GSK3A and GSK3B which facilitates RICTOR ubiquitination and subsequent degradation (PubMed:25897075). Phosphorylated at Ser-1235 by GSK3B in response to endoplasmic stress, inhibiting mTORC2 signaling (PubMed:21343617);Ubiquitinated by the SCF(FBXW7) complex, leading to its degradation by the proteasome (PubMed:25897075). Deubiquitinated by USP9X; deubiquitination stabilizes RICTOR and enhances its binding to MTOR, thus promoting mTORC2 complex assembly (PubMed:33378666);Acetylated by EP300/p300 in response to glucose, leading to activate the mTORC2 complex (PubMed:22084251, PubMed:26170313). Acetylation by BLOC1S1/GCN5L1 in response to hypotoxic stress protects RICTOR against ubiquitination and subsequent degradation by the proteasome (PubMed:38281616)
Subunit：Component of the mechanistic target of rapamycin complex 2 (mTORC2), consisting in two heterotretramers composed of MTOR, MLST8, RICTOR and MAPKAP1/SIN1 (PubMed:15268862, PubMed:15467718, PubMed:17461779, PubMed:17599906, PubMed:29424687, PubMed:29567957, PubMed:33158864, PubMed:34519268, PubMed:35926713). The mTORC2 core complex associates with PRR5/PROTOR1 and/or PRR5L/PROTOR2 (PubMed:17461779, PubMed:21964062, PubMed:29424687). Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin (PubMed:15467718, PubMed:33158864). Binds directly to MTOR and PRR5 within the TORC2 complex; interaction with MTOR is enhanced by deubiquitination of RICTOR by USP9X (PubMed:15268862, PubMed:17461779, PubMed:17599906, PubMed:33378666). Interaction with MAPKAP1 is not enhanced by RICTOR deubiquitination by USP9X (PubMed:33378666). Interacts with CCDC28B (PubMed:23727834). Interacts with NBN (PubMed:23762398). Interacts with SIK3 (PubMed:30232230). Interacts with NCKAP1L (PubMed:32647003). Interacts with kinases GSK3A and GSK3B; the interactions lead to phosphorylation of RICTOR at Thr-1695 which facilitates its FBXW7-mediated ubiquitination and subsequent degradation (PubMed:25897075). Interacts with FBXW7; the interaction is enhanced by GSK3-mediated phosphorylation of Thr-1695 and results in RICTOR ubiquitination and degradation (PubMed:25897075). Interacts with ARMH4 (via cytoplasmic tail); this interaction bridges ARMH4 to the mTORC2 complex and inhibits the mTORC2 kinase activity (By similarity). Interacts with UBXN2A (PubMed:37037900). Interacts with TSPAN8 (PubMed:25761241, PubMed:35904232)
Synonyms
Rapamycin-insensitive companion of mTOR; AVO3 homolog; hAVO3;
ドキュメンテーション
User Guide for Antibodies (1077 KB)

RICTOR Antibody (YA5903) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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RICTOR Antibody (YA5903)
製品番号:
HY-P86211
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