Occludin Antibody (YA5499)
(Synonyms: Occludin, OCLN)
Based on 1 Customer Validation
Occludin Antibody (YA5499) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Occludin.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:1000-5000 |
Product Details
Occludin Antibody (YA5499) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Occludin.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 59 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 59 kDa
AA range: 1-522
affinity chromatography.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Occludin may play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions; (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes
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Subcellular Localization
Cell membrane; Multi-pass membrane protein; Cell junction, tight junction
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Expression
Tissue_specificity:It is located at the tight junctions of epithelial and endothelial cells. It is highly expressed in the kidneys. It was not detected in the testes. -
Isoforms & Post-Translational Modification
Q16625 has 7 isomers: Q16625-1: 59144 Da (predicted); Q16625-2: 52706 Da (predicted); Q16625-3: 54124 Da (predicted); Q16625-4: 31602 Da (predicted); Q16625-5: 23324 Da (predicted); Q16625-6: 8033 Da (predicted); Q16625-7: 8175 Da (predicted).
Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1;(Microbial infection) Cleaved by S.pyogenes SpeB protease; leading to its degradation (PubMed:23532847). Degradation by SpeB promotes bacterial translocation across the host epithelial barrier (PubMed:23532847) -
Subunit
Interacts with TJP1/ZO1 (PubMed:19017651). Interacts with VAPA (PubMed:10523508). Interacts with CLDN1, CLDN6, CLDN9, CLDN11, CLDN12 and CLDN17 (PubMed:20375010). Interacts with PLSCR1 (PubMed:21806988). Interacts with LSR, ILDR1 and ILDR2 (PubMed:23239027). Interacts with TJP2/ZO2 (By similarity)
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SwissProt ID
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Synonyms
Occludin, OCLN
Documentation
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