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  4. TSPAN16 Antibody (YA4572)

TSPAN16 Antibody (YA4572)

Art. -Nr.: HY-P84875
COA User Guide for Antibodies Technical Support

TSPAN16 Antibody (YA4572) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TSPAN16.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

  • Verification Image

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  • Beschreibung

Beschreibung

TSPAN16 Antibody (YA4572) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TSPAN16.

Host

Mouse

Clonality

Monoclonal

Molekulargewicht
Predicted band size: 26 kDa;
Observed band size: 70-90 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat, Monkey
SwissProt ID
Gene ID
Immunogen

Purified recombinant fragment of human TSPAN16 (AA: 116-245) expressed in E. Coli.

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:200-1:1000
FC
FC: Flow Cytometry
1:200-1:400
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:10000
Reinheit affinity purified. Conjugation Non-conjugated
Modification Unmodified Isotype IgG1
Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image
WB
  • Western blot analysis of extracts from Raji(lane 2(20μg), HL60(lane 3(20μg), Hela (lane 4(20μg) and A549(lane 5(20μg) using TSPAN16 Antibody (HY-P84875). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Subcellular Localization:Membrane; Multi-pass membrane protein
Expression:
Tissue_specificity:Broadly expressed in most human tissues and cell lines including neural and bone marrow derived tissues
Isoforms & Post-Translational Modification:Q9UKR8 has four isomers: Q9UKR8-1: 26266 Da (predicted); Q9UKR8-2: 26058 Da (predicted); Q9UKR8-3: 23339 Da (predicted); Q9UKR8-4: 17205 Da (predicted).
RRID
Synonyms
TM-8; TM4-B; TM4SF16
Dokumentation

TSPAN16 Antibody (YA4572) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Produktname:
TSPAN16 Antibody (YA4572)
Art. -Nr.:
HY-P84875
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