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TSPAN16 Antibody (YA4572)

(Synonyms: TM-8; TM4-B; TM4SF16)
Customer Review

Based on 1 Customer Validation

TSPAN16 Antibody (YA4572) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TSPAN16.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TSPAN16 Antibody (YA4572)
  • TSPAN16 Antibody (YA4572) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

TSPAN16 Antibody (YA4572) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TSPAN16.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 70-90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 26 kDa
Immunogen

Purified recombinant fragment of human TSPAN16 (AA: 116-245) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718994

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TSPAN16 Antibody (YA4572)
    Western blot analysis of extracts from Raji(lane 2(20μg), HL60(lane 3(20μg), Hela (lane 4(20μg) and A549(lane 5(20μg) using TSPAN16 Antibody (HY-P84875). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Subcellular Localization

    Membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Broadly expressed in most human tissues and cell lines including neural and bone marrow derived tissues

  • Isoforms & Post-Translational Modification

    Q9UKR8 has four isomers: Q9UKR8-1: 26266 Da (predicted); Q9UKR8-2: 26058 Da (predicted); Q9UKR8-3: 23339 Da (predicted); Q9UKR8-4: 17205 Da (predicted).

  • SwissProt ID

    Q9UKR8

  • Gene ID
    26526 [NCBI]
  • Synonyms

    TM-8; TM4-B; TM4SF16

TSPAN16 Antibody (YA4572) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Raji(lane 2(20μg), HL60(lane 3(20μg), Hela (lane 4(20μg) and A549(lane 5(20μg) using TSPAN16 Antibody (HY-P84875). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • TSPAN16 Antibody (YA4572) Product Image
    1/16
  • Experimental Validation Results for TSPAN16 Antibody (YA4572)
  • TSPAN16 Antibody (YA4572) Product Image
  • Experimental Validation Results for TSPAN16 Antibody (YA4572)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Raji(lane 2(20μg), HL60(lane 3(20μg), Hela (lane 4(20μg) and A549(lane 5(20μg) using TSPAN16 Antibody (HY-P84875). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TSPAN16 Antibody (YA4572)
  • TSPAN16 Antibody (YA4572) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Raji(lane 2(20μg), HL60(lane 3(20μg), Hela (lane 4(20μg) and A549(lane 5(20μg) using TSPAN16 Antibody (HY-P84875). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • TSPAN16 Antibody (YA4572) Product Image WB