Copy Link Print Information

PAX5 Antibody (YA6049)

(Synonyms: Paired box protein Pax-5 (B-cell-specific transcription factor) (BSAP))
Customer Review

Based on 1 Customer Validation

PAX5 Antibody (YA6049) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAX5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PAX5 Antibody (YA6049)
  • PAX5 Antibody (YA6049) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:2000-1:10000 1:500-1:2000 1:200-1:1000 1:5000-1:20000

Product Details

Description

PAX5 Antibody (YA6049) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAX5.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PAX5 Antibody (YA6049)
    Western blot analysis of extracts from Daudi (lane 1(20μg)) 、Ramos (lane 2(20μg)) 、Mouse lymph (lane 3(20μg)) and Rat lymph (lane 4(20μg)) using PAX5 Antibody (HY-P86357) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    MEK-4 transcription factor that plays an essential role in commitment of lymphoid progenitors to the B-lymphocyte lineage. Fulfills a dual role by repressing B-lineage inappropriate genes and simultaneously activating B-lineage-specific genes. In turn, regulates cell adhesion and migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-cell receptor signaling and promotes development to the mature B-cell stage. Repression of the cohesin-release factor WAPL causes global changes of the chromosomal architecture in pro-B cells to facilitate the generation of a diverse antibody repertoire; (Microbial infection) Plays an essential role in the maintenance of Epstein-Barr virus genome copy number within the host cell by promoting EBNA1/oriP-dependent binding and transcription. Also participates in the inhibition of lytic EBV reactivation by modulating viral BZLF1 activity[1][2][3][4][5].

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    Q02548 has 11 isomers: Q02548-1: 42149 Da (predicted); Q02548-2: 38890 Da (predicted); Q02548-3: 35281 Da (predicted); Q02548-4: 32471 Da (predicted); Q02548-5: 31830 Da (predicted); Q02548-6: 38773 Da (predicted); Q02548-7: 35514 Da (predicted); Q02548-8: 37834 Da (predicted); Q02548-9: 34574 Da (predicted); Q02548-10: 31381 Da (predicted); Q02548-11: 34132 Da (predicted).
    O-glycosylated;Phosphorylated by SYK. This phosphorylation plays an important role in the abolition of BLIMP1 repression by PAX5 in order to trigger plasma cell differentiation

  • Subunit

    Interacts with ETS1; this interaction alters PAX5 DNA-binding properties (PubMed:11779502). Binds DNA as a monomer (PubMed:11779502). Interacts with TBP; this interaction allows PAX5 to interact with the basal transcription machinery (PubMed:10197586). Interacts with RB1 (PubMed:10197586). Interacts with TLE4 (PubMed:10811620). Interacts with DAXX (By similarity)

  • SwissProt ID

    Q02548

  • Gene ID
    5079 [NCBI]
  • Synonyms

    Paired box protein Pax-5 (B-cell-specific transcription factor) (BSAP)

References

[1]. Eberhard D, et al. Transcriptional repression by Pax5 (BSAP) through interaction with corepressors of the Groucho family. EMBO J. 2000 May 15;19(10):2292-303. [Content Brief]

[2]. Inagaki Y, et al. PAX5 tyrosine phosphorylation by SYK co-operatively functions with its serine phosphorylation to cancel the PAX5-dependent repression of BLIMP1: A mechanism for antigen-triggered plasma cell differentiation. Biochem Biophys Res Commun. 2016 Jun 24;475(2):176-81. [Content Brief]

[3]. Hill L, et al. Wapl repression by Pax5 promotes V gene recombination by Igh loop extrusion. Nature. 2020 Aug;584(7819):142-147. [Content Brief]

[4]. Liu CD, et al. B Cell-Specific Transcription Activator PAX5 Recruits p300 To Support EBNA1-Driven Transcription. J Virol. 2020 Mar 17;94(7):. [Content Brief]

[5]. Raver RM, et al. The B-cell-specific transcription factor and master regulator Pax5 promotes Epstein-Barr virus latency by negatively regulating the viral immediate early protein BZLF1. J Virol. 2013 Jul;87(14):8053-63. [Content Brief]

View More

PAX5 Antibody (YA6049) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Daudi (lane 1(20μg)) 、Ramos (lane 2(20μg)) 、Mouse lymph (lane 3(20μg)) and Rat lymph (lane 4(20μg)) using PAX5 Antibody (HY-P86357) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • PAX5 Antibody (YA6049) Product Image
    1/16
  • Experimental Validation Results for PAX5 Antibody (YA6049)
  • PAX5 Antibody (YA6049) Product Image
  • Experimental Validation Results for PAX5 Antibody (YA6049)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Daudi (lane 1(20μg)) 、Ramos (lane 2(20μg)) 、Mouse lymph (lane 3(20μg)) and Rat lymph (lane 4(20μg)) using PAX5 Antibody (HY-P86357) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PAX5 Antibody (YA6049)
  • PAX5 Antibody (YA6049) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Daudi (lane 1(20μg)) 、Ramos (lane 2(20μg)) 、Mouse lymph (lane 3(20μg)) and Rat lymph (lane 4(20μg)) using PAX5 Antibody (HY-P86357) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • PAX5 Antibody (YA6049) Product Image WB