BUB3 Antibody (YA2180)

Cat. No.: HY-P82435: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
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BUB3 Antibody (YA2180) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BUB3.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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BUB3 Antibody (YA2180) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

BUB3 Antibody (YA2180) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BUB3.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 37 kDa;

Observed band size: 40 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O43684

Gene ID

9184 [NCBI]

Immunogen

A synthesized peptide derived from human Bub3 aa1-50/328.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100
IP IP: Immunoprecipitation	1:50

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1

Subcellular Localization：Nucleus; Chromosome, centromere, kinetochore

Isoforms & Post-Translational Modification：O43684 has 2 isomers: O43684-1: 37155 Da (predicted); O43684-2: 36955 Da (predicted).
Poly-ADP-ribosylated by PARP1;Ubiquitinated by UBR5, promoting disassembly of the mitotic checkpoint complex from the APC/C complex

Subunit：Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Interacts with ZNF207/BuGZ; leading to promote stability and kinetochore loading of BUB3

Database

Entrez Gene: 9184 Human ; 12237 Mouse ; 361662 Rat

SwissProt: O43684 Human ; Q9WVA3 Mouse ;

OMIM: 603719 Human

Research Field

Cell Biology

Synonyms

BUB3; Mitotic checkpoint protein BUB3

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hela-cell.html [Content Brief]

BUB3 Antibody (YA2180) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BUB3 Antibody (YA2180)

BUB3 Antibody (YA2180) Featured Recommendations:

Product Detail

Background

Documentation

References

BUB3 Antibody (YA2180) Related Classifications

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