BUB3 Antibody (YA2180)
(Synonyms: BUB3; Mitotic checkpoint protein BUB3)
Based on 1 Customer Validation
BUB3 Antibody (YA2180) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BUB3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:100-1:200 | 1:50-1:200 | 1:50-1:100 | 1:50 |
Product Details
BUB3 Antibody (YA2180) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BUB3.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 40 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 37 kDa
Entrez Gene: 9184 Human ; 12237 Mouse ; 361662 Rat
SwissProt: O43684 Human ; Q9WVA3 Mouse ;
OMIM: 603719 Human
A synthesized peptide derived from human Bub3 aa1-50/328.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
BUB3 is a Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1
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Subcellular Localization
Nucleus; Chromosome, centromere, kinetochore
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Isoforms & Post-Translational Modification
O43684 has 2 isomers: O43684-1: 37155 Da (predicted); O43684-2: 36955 Da (predicted).
Poly-ADP-ribosylated by PARP1;Ubiquitinated by UBR5, promoting disassembly of the mitotic checkpoint complex from the APC/C complex -
Subunit
Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Interacts with ZNF207/BuGZ; leading to promote stability and kinetochore loading of BUB3
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SwissProt ID
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Synonyms
BUB3; Mitotic checkpoint protein BUB3
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Research Field
Cell Biology
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