Gli2 Antibody (YA6586)

Cat. No.: HY-P86893: Data Sheet COA
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Gli2 Antibody (YA6586) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Gli2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Gli2 Antibody (YA6586) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Gli2.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 168 kDa;

Observed band size: 133 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P10070

Gene ID

2736 [NCBI]

Immunogen

Recombinant protein within human Gli2 aa 571-830.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Functions as a transcription regulator in the hedgehog (Hh) pathway (PubMed:18455992, PubMed:26565916). Functions as a transcriptional activator (PubMed:19878745, PubMed:24311597, PubMed:9557682). May also function as transcriptional repressor (By similarity). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development (PubMed:15994174, PubMed:20685856)|Involved in the smoothened (SHH) signaling pathway|Involved in the smoothened (SHH) signaling pathway|Involved in the smoothened (SHH) signaling pathway|Involved in the smoothened (SHH) signaling pathway|Acts as a transcriptional activator in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1 (PubMed:9557682)|(Microbial infection) Acts as a transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1 (PubMed:9557682)|(Microbial infection) Acts as a transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1 (PubMed:9557682)|(Microbial infection) Acts as a transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1 (PubMed:9557682)|Acts as a transcriptional repressor

Subcellular Localization：Nucleus,Cytoplasm,Cell projection, cilium,Nucleus,Nucleus

Expression：
Tissue_Specificity: Expressed in breast cancers (at protein level) (PubMed:26565916). Isoform 1 and isoform 4 are expressed in HTLV-1-infected T-cell lines (at protein level) (PubMed:9557682). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines (PubMed:9557682). Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines (PubMed:9557682)

Isoforms & Post-Translational Modification：P10070 has 5 isomers: P10070-5: 167783 Da (predicted); P10070-1: 133241 Da (predicted); P10070-2: 131659 Da (predicted); P10070-3: 88065 Da (predicted); P10070-4: 86482 Da (predicted).
Phosphorylated in vitro by ULK3丨Acetylation at Lys-757 inhibits Hh target gene expression, probably by impeding entry into chromatin thus preventing promoter occupancy

Subunit：Interaction with ZIC1 and ZIC2 (By similarity)

Synonyms

CJS antibody; Gli 2 antibody; GLI family zinc finger 2 antibody; GLI Kruppel family member GLI2 antibody; GLI2 antibody; GLI2_HUMAN antibody; Glioma associated oncogene family zinc finger antibody; HPE9 antibody; Oncogene GLI2 antibody; PHS2 antibody; CJS antibody; Gli 2 antibody; GLI family zinc finger 2 antibody; GLI Kruppel family member GLI2 antibody; GLI2 antibody; GLI2_HUMAN antibody; Glioma associated oncogene family zinc finger antibody; HPE9 antibody; Oncogene GLI2 antibody; PHS2 antibody; Tax helper protein 1 antibody; Tax helper protein 2 antibody; Tax helper protein antibody; Tax responsive element 2 holding protein antibody; Tax responsive element 25 bp sequence binding protein antibody; THP antibody; THP1 antibody; THP2 antibody; Zinc finger protein GLI2 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)