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Aquaporin 5 Antibody

(Synonyms: Aquaporin 5; AQP5; AQP 5; AQP-5; AQP5_HUMAN; Aquaporin-5.)
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Based on 1 publication(s) in Google Scholar

Aquaporin 5 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Aquaporin 5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol or 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Aquaporin 5 Antibody
  • Aquaporin 5 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-2000

Product Details

Description

Aquaporin 5 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Aquaporin 5.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 27 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 29 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from mouse AQP5: 201-265/265

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103068

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol or 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Aquaporin 5 Antibody
    Western blot analysis of extracts from Mouse Lung (lane 1) using Aquaporin 5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    Aquaporin 5 is an Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient. Plays an important role in fluid secretion in salivary glands. Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells. Seems to play a redundant role in water transport in the eye, lung and in sweat glands[1][2][3][4][5].

  • Subcellular Localization

    Apical cell membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein; Cytoplasmic vesicle membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Detected at the luminal membrane of secretory epithelial cells in hindpaw sweat glands (PubMed:12042359, PubMed:23473857) . Detected in acinar cells in salivary glands, in duct cells in lacrimal glands and in lung (at protein level) (PubMed:10337625, PubMed:10400615, PubMed:10619865, PubMed:16571723, PubMed:18027168) . Detected in lung, parotid, submandibular, sublingual, and lacrimal gland tissues (PubMed:10337625)

  • Subunit

    Homotetramer; each monomer provides an independent water pore (By similarity). Interacts with TRPV4; the interaction is probably indirect and regulates TRPV4 activation by hypotonicity (PubMed:16571723)

  • SwissProt ID

    Q9WTY4

  • Gene ID
    11830 [NCBI]
  • Synonyms

    Aquaporin 5; AQP5; AQP 5; AQP-5; AQP5_HUMAN; Aquaporin-5.

Aquaporin 5 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Mouse Lung (lane 1) using Aquaporin 5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Aquaporin 5 Antibody Product Image
    1/16
  • Experimental Validation Results for Aquaporin 5 Antibody
  • Aquaporin 5 Antibody Product Image
  • Experimental Validation Results for Aquaporin 5 Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Mouse Lung (lane 1) using Aquaporin 5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Aquaporin 5 Antibody
  • Aquaporin 5 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Mouse Lung (lane 1) using Aquaporin 5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Aquaporin 5 Antibody Product Image WB