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HTR7 Antibody (YA2796)

(Synonyms: 5 HT 7; 5 HT X; 5-HT-7; 5-HT-X; 5-HT7; 5HT7; htr7)
Customer Review

Based on 1 Customer Validation

HTR7 Antibody (YA2796) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HTR7.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HTR7 Antibody (YA2796)
  • HTR7 Antibody (YA2796) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

HTR7 Antibody (YA2796) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HTR7.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human 5HT7 Receptor aa2-58/479.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105167

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HTR7 Antibody (YA2796)
    Western blot analysis of extracts from U87-MG (lane 2(20μg)), CaCo2 (lane 3(20μg)) and Rat brain (lane 4(20μg)) using 5HT7 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    HTR7 is a G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR7 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity[1][2].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Predominant isoform in spleen, caudate and hippocampus; Expressed at lower levels; Minor isoform in terms of expression

  • Isoforms & Post-Translational Modification

    P34969 has 3 isomers: P34969-1: 53555 Da (predicted); P34969-2: 49488 Da (predicted); P34969-3: 47984 Da (predicted).

  • SwissProt ID

    P34969

  • Gene ID
    3363 [NCBI]
  • Synonyms

    5 HT 7; 5 HT X; 5-HT-7; 5-HT-X; 5-HT7; 5HT7; htr7

  • Research Field

    -

HTR7 Antibody (YA2796) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from U87-MG (lane 2(20μg)), CaCo2 (lane 3(20μg)) and Rat brain (lane 4(20μg)) using 5HT7 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HTR7 Antibody (YA2796) Product Image
    1/16
  • Experimental Validation Results for HTR7 Antibody (YA2796)
  • HTR7 Antibody (YA2796) Product Image
  • Experimental Validation Results for HTR7 Antibody (YA2796)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from U87-MG (lane 2(20μg)), CaCo2 (lane 3(20μg)) and Rat brain (lane 4(20μg)) using 5HT7 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HTR7 Antibody (YA2796)
  • HTR7 Antibody (YA2796) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from U87-MG (lane 2(20μg)), CaCo2 (lane 3(20μg)) and Rat brain (lane 4(20μg)) using 5HT7 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HTR7 Antibody (YA2796) Product Image WB