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FOXF1 Antibody (YA2681)

(Synonyms: FKHL5; foxf1; FREAC1; HFH 8)
Customer Review

Based on 1 Customer Validation

FOXF1 Antibody (YA2681) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FOXF1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
  • FOXF1 Antibody (YA2681) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:100

Product Details

Description

FOXF1 Antibody (YA2681) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FOXF1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 40 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 40 kDa
Species Reactivity Database

Entrez Gene: 2294 Human

SwissProt: Q12946 Human

Immunogen

A synthesized peptide derived from human FOXF1 aa1-50/379.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105055

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FOXF1 Antibody (YA2681)
    Western blot analysis was performed on extracts from Rat lung (lane 1, 15 μg) using FOXF1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:500 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
    Western blot analysis of extracts from HEK293 (lane 2(20μg), HEK293 (lane 3(40μg), using FOXF1 Antibody (YA2681). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
    Immunocytochemistry analysis of HeLa cells labeling FOXF1 with FOXF1 Antibody (HY-P82936) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXF1 Antibody (HY-P82936) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
    Immunocytochemistry analysis of HeLa cells labeling FOXF1 with FOXF1 Antibody (HY-P82936) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXF1 Antibody (HY-P82936) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    FOXF1 is a Probable transcription activator for a number of lung-specific genes

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Expressed in lung and placenta

  • SwissProt ID

    Q12946

  • Gene ID
    2294 [NCBI]
  • Synonyms

    FKHL5; foxf1; FREAC1; HFH 8

  • Research Field

    Epigenetics and Nuclear Signaling

FOXF1 Antibody (YA2681) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Rat lung (lane 1, 15 μg) using FOXF1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:500 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), HEK293 (lane 3(40μg), using FOXF1 Antibody (YA2681). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling FOXF1 with FOXF1 Antibody (HY-P82936) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXF1 Antibody (HY-P82936) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling FOXF1 with FOXF1 Antibody (HY-P82936) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXF1 Antibody (HY-P82936) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FOXF1 Antibody (YA2681) Product Image
    1/16
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
  • FOXF1 Antibody (YA2681) Product Image
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Rat lung (lane 1, 15 μg) using FOXF1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:500 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), HEK293 (lane 3(40μg), using FOXF1 Antibody (YA2681). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling FOXF1 with FOXF1 Antibody (HY-P82936) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXF1 Antibody (HY-P82936) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling FOXF1 with FOXF1 Antibody (HY-P82936) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXF1 Antibody (HY-P82936) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
  • Experimental Validation Results for FOXF1 Antibody (YA2681)
  • FOXF1 Antibody (YA2681) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Rat lung (lane 1, 15 μg) using FOXF1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:500 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • FOXF1 Antibody (YA2681) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 2(20μg), HEK293 (lane 3(40μg), using FOXF1 Antibody (YA2681). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • FOXF1 Antibody (YA2681) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling FOXF1 with FOXF1 Antibody (HY-P82936) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXF1 Antibody (HY-P82936) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FOXF1 Antibody (YA2681) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling FOXF1 with FOXF1 Antibody (HY-P82936) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXF1 Antibody (HY-P82936) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FOXF1 Antibody (YA2681) Product Image WB
  • FOXF1 Antibody (YA2681) Product Image WB
  • FOXF1 Antibody (YA2681) Product Image ICC
  • FOXF1 Antibody (YA2681) Product Image ICC