FE65 Antibody (YA2058)

Cat. No.: HY-P82313: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
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FE65 Antibody (YA2058) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FE65.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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FE65 Antibody (YA2058) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

FE65 Antibody (YA2058) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FE65.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 77 kDa;

Observed band size: 100 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O00213

Gene ID

322 [NCBI]

Immunogen

A synthesized peptide derived from human FE65 aa650-710/710.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription coregulator that can have both coactivator and corepressor functions (PubMed:15031292, PubMed:18468999, PubMed:18922798, PubMed:25342469, PubMed:33938178). Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain (PubMed:15031292, PubMed:18468999, PubMed:18922798, PubMed:25342469). Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis (PubMed:15031292, PubMed:18468999, PubMed:18922798, PubMed:25342469). May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1 (PubMed:19234442). Required for histone H4 acetylation at double-strand breaks (DSBs) (PubMed:19234442). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity (PubMed:33938178). Functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4 (PubMed:19343227). Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s) (PubMed:19343227). Involved in hippocampal neurite branching and neuromuscular junction formation, as a result plays a role in spatial memory functioning (By similarity). Plays a role in the maintenance of lens transparency (By similarity). May play a role in muscle cell strength (By similarity). Acts as a molecular adapter that functions in neurite outgrowth by activating the RAC1-ARF6 axis upon insulin treatment (PubMed:36250347)

Subcellular Localization：Cell membrane; Cytoplasm; Nucleus; Cell projection, growth cone; Nucleus speckle

Expression：
Tissue_specificity:Highly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease; Expressed preferentially in the brain

Isoforms & Post-Translational Modification：O00213 has 6 isomers: O00213-1: 77244 Da (predicted); O00213-2: 76959 Da (predicted); O00213-3: 53927 Da (predicted); O00213-4: 49857 Da (predicted); O00213-5: 54212 Da (predicted); O00213-6: 52656 Da (predicted).
Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus (By similarity). Phosphorylated following nuclear translocation (PubMed:15031292, PubMed:18922798). Phosphorylation at Tyr-547 by ABL1 enhances transcriptional activation activity and reduces the affinity for RASD1/DEXRAS1 (PubMed:15031292, PubMed:18922798). Phosphorylated at Ser-459 by PKC upon insulin activation (PubMed:36250347);Acetylation at Lys-204 and Lys-701 by KAT5 promotes its transcription activator activity;Polyubiquitination by RNF157 leads to degradation by the proteasome (PubMed:25342469)

Subunit：Component of a complex, at least composed of APBB1, RASD1/DEXRAS1 and APP (PubMed:18468999, PubMed:18833287, PubMed:18922798). Interacts (via PID domain 2) with APP (with the intracellular domain of the amyloid-beta precursor protein) (PubMed:18468999, PubMed:18833287). Interacts (via PID domain 2) with RASD1/DEXRAS1; impairs the transcription activation activity (PubMed:18922798). Interacts (via PID domain 1) with KAT5/TIP60 (PubMed:33938178). Interacts (via the WW domain) with the proline-rich region of APBB1IP (By similarity). Interacts with TSHZ1 and TSHZ2 (By similarity). Interacts (via the WW domain) with histone H2AX (when phosphorylated on 'Tyr-142') and the proline-rich region of ENAH (PubMed:17686488). Interacts with MAPK8 (PubMed:19234442). Interacts (via PID domain 1) with TSHZ3 (via homeobox domain) (PubMed:19343227). Interacts with SET (PubMed:19343227). Found in a trimeric complex with HDAC1 and TSHZ3; the interaction between HDAC1 and APBB1 is mediated by TSHZ3 (PubMed:19343227). Interacts (via WWW domain) with NEK6 (PubMed:17512906). Interacts (via WWW domain) with ABL1 (PubMed:15031292). Interacts with RNF157 (PubMed:25342469). Interacts with ARF6 (PubMed:36250347)

Database

Entrez Gene: 322 Human

SwissProt: O00213 Human

OMIM: 602709 Human

Research Field

Neuroscience

Synonyms

APBB1; Protein Fe65; RIR

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

FE65 Antibody (YA2058) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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FE65 Antibody (YA2058)

FE65 Antibody (YA2058) Featured Recommendations:

Product Detail

Background

Documentation

FE65 Antibody (YA2058) Related Classifications

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