FE65 Antibody (YA2058)
(Synonyms: APBB1; Protein Fe65; RIR)
Based on 1 Customer Validation
FE65 Antibody (YA2058) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FE65.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human
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Formulation:
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50-1:200 |
Product Details
FE65 Antibody (YA2058) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FE65.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 100 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 77 kDa
A synthesized peptide derived from human FE65 aa650-710/710.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
FE65 transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity. Functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Involved in hippocampal neurite branching and neuromuscular junction formation, as a result plays a role in spatial memory functioning. Plays a role in the maintenance of lens transparency. May play a role in muscle cell strength. Acts as a molecular adapter that functions in neurite outgrowth by activating the RAC1-ARF6 axis upon insulin treatment[1][2][3][4][5][6][7][8].
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Subcellular Localization
Cell membrane; Cytoplasm; Nucleus; Cell projection, growth cone; Nucleus speckle
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Expression
Tissue_specificity:Highly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease; Expressed preferentially in the brain -
Isoforms & Post-Translational Modification
O00213 has 6 isomers: O00213-1: 77244 Da (predicted); O00213-2: 76959 Da (predicted); O00213-3: 53927 Da (predicted); O00213-4: 49857 Da (predicted); O00213-5: 54212 Da (predicted); O00213-6: 52656 Da (predicted).
Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus (By similarity). Phosphorylated following nuclear translocation (PubMed:15031292, PubMed:18922798). Phosphorylation at Tyr-547 by ABL1 enhances transcriptional activation activity and reduces the affinity for RASD1/DEXRAS1 (PubMed:15031292, PubMed:18922798). Phosphorylated at Ser-459 by PKC upon insulin activation (PubMed:36250347);Acetylation at Lys-204 and Lys-701 by KAT5 promotes its transcription activator activity;Polyubiquitination by RNF157 leads to degradation by the proteasome (PubMed:25342469) -
Subunit
Component of a complex, at least composed of APBB1, RASD1/DEXRAS1 and APP (PubMed:18468999, PubMed:18833287, PubMed:18922798). Interacts (via PID domain 2) with APP (with the intracellular domain of the amyloid-beta precursor protein) (PubMed:18468999, PubMed:18833287). Interacts (via PID domain 2) with RASD1/DEXRAS1; impairs the transcription activation activity (PubMed:18922798). Interacts (via PID domain 1) with KAT5/TIP60 (PubMed:33938178). Interacts (via the WW domain) with the proline-rich region of APBB1IP (By similarity). Interacts with TSHZ1 and TSHZ2 (By similarity). Interacts (via the WW domain) with histone H2AX (when phosphorylated on 'Tyr-142') and the proline-rich region of ENAH (PubMed:17686488). Interacts with MAPK8 (PubMed:19234442). Interacts (via PID domain 1) with TSHZ3 (via homeobox domain) (PubMed:19343227). Interacts with SET (PubMed:19343227). Found in a trimeric complex with HDAC1 and TSHZ3; the interaction between HDAC1 and APBB1 is mediated by TSHZ3 (PubMed:19343227). Interacts (via WWW domain) with NEK6 (PubMed:17512906). Interacts (via WWW domain) with ABL1 (PubMed:15031292). Interacts with RNF157 (PubMed:25342469). Interacts with ARF6 (PubMed:36250347)
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SwissProt ID
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Synonyms
APBB1; Protein Fe65; RIR
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Research Field
Neuroscience
Documentation
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Data Sheet (234 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
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