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SLC7A3 Antibody (YA4391)

(Synonyms: CAT3; ATRC3; CAT-3)
Customer Review

Based on 1 Customer Validation

SLC7A3 Antibody (YA4391) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SLC7A3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • SLC7A3 Antibody (YA4391) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

SLC7A3 Antibody (YA4391) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SLC7A3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 67 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 67.1 kDa
Immunogen

Purified recombinant fragment of human SLC7A3 (AA: extra mix) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718965

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    Western blot analysis of extracts from HT-29(lane2(20μg), A549(lane3(20μg), HCT-116(lane4(20μg) and NIH/3T3(lane5(20μg) using SLC7A3 Antibody (YA4391)(HY-P84694). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    Immunohistochemical analysis of paraffin-embedded human Brain tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    Immunohistochemical analysis of paraffin-embedded human Brain tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    Immunohistochemical analysis of paraffin-embedded human Cervix tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    Immunohistochemical analysis of paraffin-embedded human Cervix‌ tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    SLC7A3 plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases

  • Subcellular Localization

    Mitochondrion outer membrane; Single-pass membrane protein

  • Expression


    Tissue_specificity:This gene is expressed in a variety of tissues, with the highest expression levels in the heart and skeletal muscle.

  • Isoforms & Post-Translational Modification

    Q16611 has 2 isomers: Q16611-1: 23409 Da (predicted); Q16611-2: 16872 Da (predicted).

  • Subunit

    Homodimer. Formation of the homodimer is zinc-dependent (PubMed:17157251). Forms heterodimers with BCL2 and BCL2L1 isoform Bcl-X(L) (PubMed:9020082). Forms heterooligomers with BAX (PubMed:29531808). Interacts with BCL2A1 (By similarity). Interacts with RTL10/BOP (PubMed:23055042). Interacts with VDAC1 (PubMed:25296756). Interacts with GIMAP3/IAN4 and GIMAP5/IAN5 (PubMed:16509771)

  • SwissProt ID

    Q8WY07

  • Gene ID
    84889 [NCBI]
  • Synonyms

    CAT3; ATRC3; CAT-3

SLC7A3 Antibody (YA4391) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HT-29(lane2(20μg), A549(lane3(20μg), HCT-116(lane4(20μg) and NIH/3T3(lane5(20μg) using SLC7A3 Antibody (YA4391)(HY-P84694). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervix tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervix‌ tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SLC7A3 Antibody (YA4391) Product Image
    1/16
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • SLC7A3 Antibody (YA4391) Product Image
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HT-29(lane2(20μg), A549(lane3(20μg), HCT-116(lane4(20μg) and NIH/3T3(lane5(20μg) using SLC7A3 Antibody (YA4391)(HY-P84694). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervix tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervix‌ tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • Experimental Validation Results for SLC7A3 Antibody (YA4391)
  • SLC7A3 Antibody (YA4391) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HT-29(lane2(20μg), A549(lane3(20μg), HCT-116(lane4(20μg) and NIH/3T3(lane5(20μg) using SLC7A3 Antibody (YA4391)(HY-P84694). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • SLC7A3 Antibody (YA4391) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SLC7A3 Antibody (YA4391) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervical cancer tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SLC7A3 Antibody (YA4391) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Brain tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SLC7A3 Antibody (YA4391) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Brain tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SLC7A3 Antibody (YA4391) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervix tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SLC7A3 Antibody (YA4391) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervix‌ tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SLC7A3 Antibody (YA4391) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SLC7A3 Antibody (YA4391) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SLC7A3 Antibody (YA4391) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SLC7A3 Antibody (YA4391) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SLC7A3 Antibody (YA4391) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SLC7A3 Antibody (YA4391) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SLC7A3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84694, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SLC7A3 Antibody (YA4391) Product Image WB
  • SLC7A3 Antibody (YA4391) Product Image IHC-P
  • SLC7A3 Antibody (YA4391) Product Image IHC-P
  • SLC7A3 Antibody (YA4391) Product Image IHC-P
  • SLC7A3 Antibody (YA4391) Product Image IHC-P
  • SLC7A3 Antibody (YA4391) Product Image IHC-P
  • SLC7A3 Antibody (YA4391) Product Image IHC-P
  • SLC7A3 Antibody (YA4391) Product Image IHC-P
  • SLC7A3 Antibody (YA4391) Product Image IHC-P
  • SLC7A3 Antibody (YA4391) Product Image IHC-P
  • SLC7A3 Antibody (YA4391) Product Image IHC-P
  • SLC7A3 Antibody (YA4391) Product Image IHC-P
  • SLC7A3 Antibody (YA4391) Product Image IHC-P