Myelin Basic Protein Antibody (YA3271)(PBS only)
(Synonyms: Myelin membrane encephalitogenic protein; Myelin A1 protein)Myelin Basic Protein Antibody (YA3271) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myelin Basic Protein.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Rat
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
Myelin Basic Protein Antibody (YA3271) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myelin Basic Protein.
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Host Rabbit
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 18 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 33 kDa
Recombinant protein of human Myelin Basic Protein
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Myelin Basic Protein is a The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation
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Subcellular Localization
Myelin membrane; Peripheral membrane protein; Cytoplasmic side; Nucleus
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Expression
Tissue_specificity:MBP isoforms are found in the central and peripheral nervous systems, while the Golli-MBP isoform is expressed in the fetal thymus, spleen, spinal cord, and cell lines derived from the immune system. -
Isoforms & Post-Translational Modification
P02686 has 6 isomers: P02686-1: 33117 Da (predicted); P02686-2: 21522 Da (predicted); P02686-3: 21493 Da (predicted); P02686-4: 20246 Da (predicted); P02686-5: 18591 Da (predicted); P02686-6: 17343 Da (predicted).
Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic;The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6);Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated;Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells;Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1 -
Subunit
Homodimer. Isoform 3 exists as a homodimer
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SwissProt ID
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Synonyms
Myelin membrane encephalitogenic protein; Myelin A1 protein
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Research Field
Neuroscience
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