Phospho-Hormone Sensitive Lipase (Ser855) Antibody (YA2599)
(Synonyms: LIPE; Hormone-sensitive lipase; HSL)Phospho-Hormone Sensitive Lipase (Ser855) Antibody (YA2599) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Hormone Sensitive Lipase (Ser855).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
Phospho-Hormone Sensitive Lipase (Ser855) Antibody (YA2599) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Hormone Sensitive Lipase (Ser855).
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 83 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 117 kDa
A synthetic phosphopeptide corresponding to residues surrounding Ser855 of human Hormone sensitive lipase/HSL aa849-862.
Endogenous
Affinity Purified
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Hormone Sensitive Lipase is a Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs. Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production[1][2][3][4].
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Subcellular Localization
Cell membrane; Membrane, caveola; Cytoplasm, cytosol; Lipid droplet
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Expression
Tissue_specificity:testis -
Isoforms & Post-Translational Modification
Q05469 has 2 isomers: Q05469-1: 116598 Da (predicted); Q05469-2: 84128 Da (predicted).
Phosphorylation by AMPK reduces its translocation towards the lipid droplets -
Subunit
Monomer and homodimer (By similarity). Interacts with CAVIN1 in the adipocyte cytoplasm (PubMed:17026959). Interacts with PLIN5 (By similarity)
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SwissProt ID
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Synonyms
LIPE; Hormone-sensitive lipase; HSL
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Research Field
Cardiovascular
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