PHD1 Antibody (YA3325)
(Synonyms: Estrogen-induced tag 6; HPH-3; PHD1)PHD1 Antibody (YA3325) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHD1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
PHD1 Antibody (YA3325) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHD1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 44 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 44 kDa
A synthesized peptide derived from human PHD1/prolyl hydroxylase aa21-34.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PHD1 is a HIF prolyl hydroxylase that regulates HIF-1 protein levels in mammalian cells. HIF-1α prolyl hydroxylases mainly include PHD1, PHD2, and PHD3. These three enzymes have sequence-similar catalytic domains and conserve amino acid residues for coordinating Fe (II) ions. Under hypoxic conditions, due to insufficient oxygen supply, the hydroxylase activity of PHD1 decreases, leading to HIF-1α accumulation and inducing the expression of HRE-regulated target genes. Therefore, PHDs are considered intracellular oxygen sensors. PHD1 is found only in the cell nucleus, while PHD2 is mainly located in the cytoplasm.
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:Expressed in human heart, brain, liver, lung, skeletal muscle, and kidney. Also expressed in testis and placenta. Highest levels in human brain, placenta, lung, kidney, and testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium
Induction:By estrogen (PubMed:11850811) . Induced by proteasomal inhibitor MG132 (at protein level) (PubMed:16509823) ; Induced by hypoxia leading to protein stability; Repressed by hypoxia -
Isoforms & Post-Translational Modification
Q96KS0 has 2 isomers: Q96KS0-1: 43650 Da (predicted); Q96KS0-2: 40168 Da (predicted).
Ubiquitinated by SIAH1 and/or SIAH2 in response to the unfolded protein response (UPR), leading to its degradation -
Subunit
Interacts (preferably isoform p40) with SIAH2; the interaction targets both SIAH2 isoforms for proteasomal degradation in vitro (PubMed:16509823). Interacts with LIMD1, WTIP and AJUBA (PubMed:22286099)
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SwissProt ID
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Synonyms
Estrogen-induced tag 6; HPH-3; PHD1
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Research Field
Cardiovascular
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