Rad50 Antibody (YA1681)
(Synonyms: RAD50; DNA repair protein RAD50; hRAD50)Rad50 Antibody (YA1681) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Rad50.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
Rad50 Antibody (YA1681) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Rad50.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 154 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 154 kDa
A synthetic peptide of human Rad50
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Rad50 component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination. Within the complex, RAD50 is both required to bind DNA ends and hold them in close proximity and regulate the activity of MRE11. RAD50 provides an ATP-dependent control of MRE11 by positioning DNA ends into the MRE11 active site: ATP-binding induces a large structural change from an open form with accessible MRE11 nuclease sites into a closed form. The MRN complex is also required for DNA damage signaling via activation of the ATM and ATR kinases: the nuclease activity of MRE11 is not required to activate ATM and ATR. The MRN complex is also required for the processing of R-loops. In telomeres the MRN complex may modulate t-loop formation[1][2][3][4][5][6][7][8][9][10][11][12][13][14].
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Subcellular Localization
Nucleus; Chromosome, telomere; Chromosome
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Expression
Tissue_specificity:Except in the testes, this gene is expressed at extremely low levels in most tissues, but at relatively high levels in the testes. It is also expressed in fibroblasts. -
Isoforms & Post-Translational Modification
Q92878 has 3 isomers: Q92878-1: 153892 Da (predicted); Q92878-2: 154587 Da (predicted); Q92878-3: 138432 Da (predicted).
Phosphorylation at Ser-635 by ATM in response to DNA damage is required for double-strand break (DSB) repair -
Subunit
Component of the MRN complex composed of two heterodimers RAD50 and MRE11 associated with a single NBN (PubMed:10839544, PubMed:26215093, PubMed:28867292, PubMed:36577401, PubMed:8756642, PubMed:9590181, PubMed:9705271).
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SwissProt ID
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Synonyms
RAD50; DNA repair protein RAD50; hRAD50
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Research Field
Epigenetics and Nuclear Signaling
Documentation
References
[1]. Lee JH, et al. Direct activation of the ATM protein kinase by the Mre11/Rad50/Nbs1 complex. Science. 2004 Apr 2;304(5667):93-6. [Content Brief]
[2]. Gatei M, et al. ATM protein-dependent phosphorylation of Rad50 protein regulates DNA repair and cell cycle control. J Biol Chem. 2011 Sep 9;286(36):31542-56. [Content Brief]
[3]. Anand R, et al. Phosphorylated CtIP Functions as a Co-factor of the MRE11-RAD50-NBS1 Endonuclease in DNA End Resection. Mol Cell. 2016 Dec 1;64(5):940-950. [Content Brief]
[4]. Park YB, et al. Eukaryotic Rad50 functions as a rod-shaped dimer. Nat Struct Mol Biol. 2017 Mar;24(3):248-257. [Content Brief]
[5]. Myler LR, et al. Single-Molecule Imaging Reveals How Mre11-Rad50-Nbs1 Initiates DNA Break Repair. Mol Cell. 2017 Sep 7;67(5):891-898.e4. [Content Brief]
[6]. Carney JP, et al. The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response. Cell. 1998 May 1;93(3):477-86. [Content Brief]
[7]. Paull TT, et al. The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks. Mol Cell. 1998 Jun;1(7):969-79. [Content Brief]
[8]. Trujillo KM, et al. Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95. J Biol Chem. 1998 Aug 21;273(34):21447-50. [Content Brief]
[9]. de Jager M, et al. Human Rad50/Mre11 is a flexible complex that can tether DNA ends. Mol Cell. 2001 Nov;8(5):1129-35. [Content Brief]
[10]. van Noort J, et al. The coiled-coil of the human Rad50 DNA repair protein contains specific segments of increased flexibility. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7581-6. [Content Brief]
[11]. Lee JH, et al. ATM activation by DNA double-strand breaks through the Mre11-Rad50-Nbs1 complex. Science. 2005 Apr 22;308(5721):551-4. [Content Brief]
[12]. Dupré A, et al. Two-step activation of ATM by DNA and the Mre11-Rad50-Nbs1 complex. Nat Struct Mol Biol. 2006 May;13(5):451-7. [Content Brief]
[13]. Chang EY, et al. MRE11-RAD50-NBS1 promotes Fanconi Anemia R-loop suppression at transcription-replication conflicts. Nat Commun. 2019 Sep 19;10(1):4265. [Content Brief]
[14]. Zhu XD, et al. Cell-cycle-regulated association of RAD50/MRE11/NBS1 with TRF2 and human telomeres. Nat Genet. 2000 Jul;25(3):347-52. [Content Brief]
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