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IGFBP7 Antibody (YA3079)

(Synonyms: IGFBP7; MAC25; PSF; Insulin-like growth factor-binding protein 7; IBP-7; IGF-binding protein 7; IGFBP-7; IGFBP-rP1; MAC25 protein; PGI2-stimulating factor; Prostacyclin-stimulating factor; Tumor-derived adhesion factor; TAF)
Customer Review

Based on 1 Customer Validation

IGFBP7 Antibody (YA3079) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP7.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • IGFBP7 Antibody (YA3079) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

IGFBP7 Antibody (YA3079) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP7.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 29 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 29 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human IGFBP7

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105447

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
    Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), and MCF-7 (lane 2, 15 μg) using IGFBP7 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
    Western blot analysis of extracts from K562 (lane 2(20μg) and HEK293T (lane 3(20μg), MCF-7 (lane 4(20μg) using IGFBP7 (HY-P83334) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue using IGFBP7 Antibody (YA3079). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83334, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue using IGFBP7 Antibody (YA3079). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83334, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
    Immunocytochemistry analysis of Hela cells labeling IGFBP7 with IGFBP7 Antibody (HY-P83334) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGFBP7 Antibody ((HY-P83334) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
    Immunocytochemistry analysis of Hela cells labeling IGFBP7 with IGFBP7 Antibody (HY-P83334) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGFBP7 Antibody ((HY-P83334) at 1/100dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    IGFBP7 binds IGF1 and IGF2 with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. Acts as a ligand for CD93 to play a role in angiogenesis[1].

  • Subcellular Localization

    Secreted

  • Isoforms & Post-Translational Modification

    Q16270 has 2 isomers: Q16270-1: 29130 Da (predicted); Q16270-2: 28860 Da (predicted).
    N-glycosylated

  • Subunit

    May interact with VPS24/CHMP3; the relevance of such interaction however remains unclear. Interacts with CD93; this interaction plays a role in endothelial cells angiogenesis (PubMed:38218180)

  • SwissProt ID

    Q16270

  • Gene ID
    3490 [NCBI]
  • Synonyms

    IGFBP7; MAC25; PSF; Insulin-like growth factor-binding protein 7; IBP-7; IGF-binding protein 7; IGFBP-7; IGFBP-rP1; MAC25 protein; PGI2-stimulating factor; Prostacyclin-stimulating factor; Tumor-derived adhesion factor; TAF

  • Research Field

    Signal Transduction

IGFBP7 Antibody (YA3079) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), and MCF-7 (lane 2, 15 μg) using IGFBP7 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg) and HEK293T (lane 3(20μg), MCF-7 (lane 4(20μg) using IGFBP7 (HY-P83334) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue using IGFBP7 Antibody (YA3079). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83334, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue using IGFBP7 Antibody (YA3079). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83334, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling IGFBP7 with IGFBP7 Antibody (HY-P83334) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGFBP7 Antibody ((HY-P83334) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling IGFBP7 with IGFBP7 Antibody (HY-P83334) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGFBP7 Antibody ((HY-P83334) at 1/100dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IGFBP7 Antibody (YA3079) Product Image
    1/16
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • IGFBP7 Antibody (YA3079) Product Image
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), and MCF-7 (lane 2, 15 μg) using IGFBP7 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg) and HEK293T (lane 3(20μg), MCF-7 (lane 4(20μg) using IGFBP7 (HY-P83334) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue using IGFBP7 Antibody (YA3079). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83334, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue using IGFBP7 Antibody (YA3079). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83334, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling IGFBP7 with IGFBP7 Antibody (HY-P83334) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGFBP7 Antibody ((HY-P83334) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling IGFBP7 with IGFBP7 Antibody (HY-P83334) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGFBP7 Antibody ((HY-P83334) at 1/100dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • Experimental Validation Results for IGFBP7 Antibody (YA3079)
  • IGFBP7 Antibody (YA3079) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), and MCF-7 (lane 2, 15 μg) using IGFBP7 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • IGFBP7 Antibody (YA3079) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg) and HEK293T (lane 3(20μg), MCF-7 (lane 4(20μg) using IGFBP7 (HY-P83334) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • IGFBP7 Antibody (YA3079) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue using IGFBP7 Antibody (YA3079). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83334, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IGFBP7 Antibody (YA3079) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue using IGFBP7 Antibody (YA3079). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83334, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IGFBP7 Antibody (YA3079) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling IGFBP7 with IGFBP7 Antibody (HY-P83334) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGFBP7 Antibody ((HY-P83334) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IGFBP7 Antibody (YA3079) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling IGFBP7 with IGFBP7 Antibody (HY-P83334) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGFBP7 Antibody ((HY-P83334) at 1/100dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IGFBP7 Antibody (YA3079) Product Image WB
  • IGFBP7 Antibody (YA3079) Product Image WB
  • IGFBP7 Antibody (YA3079) Product Image IHC-P
  • IGFBP7 Antibody (YA3079) Product Image IHC-P
  • IGFBP7 Antibody (YA3079) Product Image ICC
  • IGFBP7 Antibody (YA3079) Product Image ICC