Ubiquilin 4 Antibody
(Synonyms: C1orf6, CIP75, UBIN, UBQLN4, Ubiquilin-4, Ataxin-1 interacting ubiquitin-like protein, Ataxin-1 ubiquitin-like-interacting protein A1U, Connexin43-interacting protein of 75 kDa, A1Up)Ubiquilin 4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Ubiquilin 4.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
Ubiquilin 4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Ubiquilin 4.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 75 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 63 kDa
Synthetic peptide corresponding to the center region of human Ubiquilin 4.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Ubiquilin 4 is a Regulator of protein degradation that mediates the proteasomal targeting of misfolded, mislocalized or accumulated proteins. Acts by binding polyubiquitin chains of target proteins via its UBA domain and by interacting with subunits of the proteasome via its ubiquitin-like domain. Key regulator of DNA repair that represses homologous recombination repair: in response to DNA damage, recruited to sites of DNA damage following phosphorylation by ATM and acts by binding and removing ubiquitinated MRE11 from damaged chromatin, leading to MRE11 degradation by the proteasome. MRE11 degradation prevents homologous recombination repair, redirecting double-strand break repair toward non-homologous end joining (NHEJ). Specifically recognizes and binds mislocalized transmembrane-containing proteins and targets them to proteasomal degradation. Collaborates with DESI1/POST in the export of ubiquitinated proteins from the nucleus to the cytoplasm. Also plays a role in the regulation of the proteasomal degradation of non-ubiquitinated GJA1 (By similarity). Acts as an adapter protein that recruits UBQLN1 to the autophagy machinery. Mediates the association of UBQLN1 with autophagosomes and the autophagy-related protein LC3 (MAP1LC3A/B/C) and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion[1][2][3][4][5].
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Subcellular Localization
Nucleus; Cytoplasm; Chromosome; Endoplasmic reticulum; Cytoplasm, perinuclear region; Cytoplasmic vesicle, autophagosome
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Expression
Tissue_Specificity: Highly expressed in pancreas, kidney, skeletal muscle, heart and throughout the brain, and at lower levels in placenta, lung and liver.
Induction: Up-regulated in aggressive tumors: expression is significantly increased in stage 3 and 4 neuroblastomas, compared to stage 1 disease. -
Isoforms & Post-Translational Modification
Ubiquilin 4 has 2 isoforms, Q9NRR5-1: amino acid length is 601, molecular weight is 63853 Da (predicted); Q9NRR5-2: amino acid length is 226, molecular weight is 24527 Da (predicted).
Phosphorylated by ATM at Ser-318 in response to DNA damage, leading to localization in the nucleus and recruitment to sites of DNA damage. -
Subunit
Homooligomer.
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SwissProt ID
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Synonyms
C1orf6, CIP75, UBIN, UBQLN4, Ubiquilin-4, Ataxin-1 interacting ubiquitin-like protein, Ataxin-1 ubiquitin-like-interacting protein A1U, Connexin43-interacting protein of 75 kDa, A1Up
Documentation
References
[1]. Riley BE, et al. The effects of the polyglutamine repeat protein ataxin-1 on the UbL-UBA protein A1Up. J Biol Chem. 2004 Oct 1;279(40):42290-301. [Content Brief]
[2]. Suzuki R, et al. UBQLN4 recognizes mislocalized transmembrane domain proteins and targets these to proteasomal degradation. EMBO Rep. 2016 Jun;17(6):842-57. [Content Brief]
[3]. Hirayama S, et al. Nuclear export of ubiquitinated proteins via the UBIN-POST system. Proc Natl Acad Sci U S A. 2018 May 1;115(18):E4199-E4208. [Content Brief]
[4]. Jachimowicz RD, et al. UBQLN4 Represses Homologous Recombination and Is Overexpressed in Aggressive Tumors. Cell. 2019 Jan 24;176(3):505-519.e22. [Content Brief]
[5]. Lee DY, et al. Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery. EMBO Rep. 2013 Apr;14(4):373-81. [Content Brief]
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