Haptoglobin Antibody (YA2545)

Cat. No.: HY-P82800: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Haptoglobin Antibody (YA2545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Haptoglobin.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Haptoglobin Antibody (YA2545) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Haptoglobin Antibody (YA2545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Haptoglobin.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 45 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P00738

Gene ID

3240 [NCBI]

Immunogen

A synthesized peptide derived from human Haptoglobin aa390-406.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), L929 (lane 4(20μg) and 3T3-L1 (lane 5(20μg) using Haptoglobin Antibody (HY-P82800) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway; The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens

Subcellular Localization：Secreted

Expression：
Tissue_specificity:Expressed by the liver and secreted in plasma

Isoforms & Post-Translational Modification：P00738 has 2 isomers: P00738-1: 45205 Da (predicted); P00738-2: 38452 Da (predicted).

Subunit：Tetramer of two alpha and two beta chains; disulfide-linked (PubMed:4573324, PubMed:6997877). Interacts with ERGIC3 (PubMed:31142615)

RRID

AB_3104894

Database

Entrez Gene: 3240 Human ; 15439 Mouse ; 24464 Rat

SwissProt: P00738 Human ; Q61646 Mouse ; P06866 Rat

OMIM: 614081 Human

Research Field

Cardiovascular

Synonyms

Binding peptide; Haptoglobin alpha chain; Haptoglobin beta chain; HP2ALPHA2; HPA1S; Zonulin

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Haptoglobin Antibody (YA2545) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Haptoglobin Antibody (YA2545)

Haptoglobin Antibody (YA2545) Featured Recommendations:

Product Detail

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Background

Documentation

Haptoglobin Antibody (YA2545) Related Classifications

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