Haptoglobin Antibody (YA2545)

製品番号: HY-P82800: データシート SDS COA User Guide for Antibodies
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Haptoglobin Antibody (YA2545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Haptoglobin.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Haptoglobin Antibody (YA2545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Haptoglobin.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 45 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P00738

Gene ID

3240 [NCBI]

Immunogen

A synthesized peptide derived from human Haptoglobin aa390-406.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	純度	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), L929 (lane 4(20μg) and 3T3-L1 (lane 5(20μg) using Haptoglobin Antibody (HY-P82800) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway; The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens

Subcellular Localization：Secreted

Expression：
Tissue_specificity:Expressed by the liver and secreted in plasma

Isoforms & Post-Translational Modification：P00738 has 2 isomers: P00738-1: 45205 Da (predicted); P00738-2: 38452 Da (predicted).

Subunit：Tetramer of two alpha and two beta chains; disulfide-linked (PubMed:4573324, PubMed:6997877). Interacts with ERGIC3 (PubMed:31142615)

RRID

AB_3104894

Database

Entrez Gene: 3240 Human ; 15439 Mouse ; 24464 Rat

SwissProt: P00738 Human ; Q61646 Mouse ; P06866 Rat

OMIM: 614081 Human

Research Field

Cardiovascular

Synonyms

Binding peptide; Haptoglobin alpha chain; Haptoglobin beta chain; HP2ALPHA2; HPA1S; Zonulin

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)