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Haptoglobin Antibody (YA2545)

(Synonyms: Binding peptide; Haptoglobin alpha chain; Haptoglobin beta chain; HP2ALPHA2; HPA1S; Zonulin)
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Based on 1 Customer Validation

Haptoglobin Antibody (YA2545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Haptoglobin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Haptoglobin Antibody (YA2545)
  • Haptoglobin Antibody (YA2545) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Haptoglobin Antibody (YA2545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Haptoglobin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Haptoglobin aa390-406.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104894

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Haptoglobin Antibody (YA2545)
    Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), L929 (lane 4(20μg) and 3T3-L1 (lane 5(20μg) using Haptoglobin Antibody (HY-P82800) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Haptoglobin is an As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway; The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Expressed by the liver and secreted in plasma

  • Isoforms & Post-Translational Modification

    P00738 has 2 isomers: P00738-1: 45205 Da (predicted); P00738-2: 38452 Da (predicted).

  • Subunit

    Tetramer of two alpha and two beta chains; disulfide-linked (PubMed:4573324, PubMed:6997877). Interacts with ERGIC3 (PubMed:31142615)

  • SwissProt ID

    P00738

  • Gene ID
    3240 [NCBI]
  • Synonyms

    Binding peptide; Haptoglobin alpha chain; Haptoglobin beta chain; HP2ALPHA2; HPA1S; Zonulin

  • Research Field

    Cardiovascular

Haptoglobin Antibody (YA2545) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), L929 (lane 4(20μg) and 3T3-L1 (lane 5(20μg) using Haptoglobin Antibody (HY-P82800) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Haptoglobin Antibody (YA2545) Product Image
    1/16
  • Experimental Validation Results for Haptoglobin Antibody (YA2545)
  • Haptoglobin Antibody (YA2545) Product Image
  • Experimental Validation Results for Haptoglobin Antibody (YA2545)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), L929 (lane 4(20μg) and 3T3-L1 (lane 5(20μg) using Haptoglobin Antibody (HY-P82800) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Haptoglobin Antibody (YA2545)
  • Haptoglobin Antibody (YA2545) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg), Hela (lane 3(20μg), L929 (lane 4(20μg) and 3T3-L1 (lane 5(20μg) using Haptoglobin Antibody (HY-P82800) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Haptoglobin Antibody (YA2545) Product Image WB