SCOT Antibody
(Synonyms: OXCT, SCOT, OXCT1, 3-oxoacid CoA-transferase 1, Somatic-type succinyl-CoA:3-oxoacid CoA-transferase, Succinyl-CoA:3-oxoacid CoA transferase, SCOT-s)SCOT Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to SCOT.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IHC-P
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
SCOT Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to SCOT.
-
Host Rabbit
-
Clonality Polyclonal
-
Species ReactivityHuman, Mouse, Rat
-
Observed Molecular WeightObserved band size: 56 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 13; 56 kDa
Purified recombinant protein of human SCOT
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
SCOT is key enzyme for ketone body catabolism. Catalyzes the first, rate-limiting step of ketone body utilization in extrahepatic tissues, by transferring coenzyme A (CoA) from a donor thiolester species (succinyl-CoA) to an acceptor carboxylate (acetoacetate), and produces acetoacetyl-CoA. Acetoacetyl-CoA is further metabolized by acetoacetyl-CoA thiolase into two acetyl-CoA molecules which enter the citric acid cycle for energy production. Forms a dimeric enzyme where both of the subunits are able to form enzyme-CoA thiolester intermediates, but only one subunit is competent to transfer the CoA moiety to the acceptor carboxylate (3-oxo acid) and produce a new acyl-CoA. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (By similarity)[1].
-
Subcellular Localization
Mitochondrion
-
Expression
Tissue_Specificity: Abundant in heart, followed in order by brain, kidney, skeletal muscle, and lung, whereas in liver it is undetectable. Expressed (at protein level) in all tissues (except in liver), most abundant in myocardium, then brain, kidney, adrenal glands, skeletal muscle and lung; also detectable in leukocytes and fibroblasts. -
Isoforms & Post-Translational Modification
SCOT has 2 isoforms, P55809-1: amino acid length is 520, molecular weight is 56158 Da (predicted); P55809-2: amino acid length is 123, molecular weight is 13358 Da (predicted).SCOT 存在 2 个异构体，P55809-1：氨基酸个数为 520 个，分子量为 56158 Da (预测)；P55809-2：氨基酸个数为 123 个，分子量为 13358 Da (预测)。
-
Subunit
Homodimer.
-
SwissProt ID
-
Synonyms
OXCT, SCOT, OXCT1, 3-oxoacid CoA-transferase 1, Somatic-type succinyl-CoA:3-oxoacid CoA-transferase, Succinyl-CoA:3-oxoacid CoA transferase, SCOT-s
Documentation
Powered by Bioz
