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TRAM2 Antibody (YA2825)

(Synonyms: KIAA0057; TRAM like protein; Tram2; Translocating chain associating membrane protein 2; Translocation associated membrane protein 2)
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Based on 1 Customer Validation

TRAM2 Antibody (YA2825) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRAM2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TRAM2 Antibody (YA2825)
  • TRAM2 Antibody (YA2825) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

TRAM2 Antibody (YA2825) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRAM2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human TRAM2 aa250-300/370.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105196

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TRAM2 Antibody (YA2825)
    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), U2OS (lane 2, 15 μg), RAW264.7 (lane 3, 15 μg), U-87MG (lane 4, 15 μg), HT-1080 (lane 5, 15 μg), and U251 (lane 6, 15 μg) using TRAM2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:90000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    TRAM2 is an E3 ubiquitin-protein ligase that regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Catalyzes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, IKBKE, MLST8, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE. Acts as a regulator of mTORC1 and mTORC2 assembly by mediating 'Lys-63'-linked ubiquitination of MLST8, thereby inhibiting formation of the mTORC2 complex, while facilitating assembly of the mTORC1 complex. Required for normal antibody isotype switching from IgM to IgG[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Isomer 1 and isomer 2 are expressed in the spleen, adipose tissue, skeletal muscle, thymus, testes, heart, lungs, and brain. Isomer 2 is expressed very weakly in the heart, lungs, and brain.

  • Subunit

    Homotrimer (By similarity). Heterotrimer with TRAF1 (PubMed:8069916).

  • SwissProt ID

    Q15035

  • Gene ID
    7186 [NCBI]
  • Synonyms

    KIAA0057; TRAM like protein; Tram2; Translocating chain associating membrane protein 2; Translocation associated membrane protein 2

  • Research Field

    Signal Transduction

TRAM2 Antibody (YA2825) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), U2OS (lane 2, 15 μg), RAW264.7 (lane 3, 15 μg), U-87MG (lane 4, 15 μg), HT-1080 (lane 5, 15 μg), and U251 (lane 6, 15 μg) using TRAM2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:90000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TRAM2 Antibody (YA2825) Product Image
    1/16
  • Experimental Validation Results for TRAM2 Antibody (YA2825)
  • TRAM2 Antibody (YA2825) Product Image
  • Experimental Validation Results for TRAM2 Antibody (YA2825)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), U2OS (lane 2, 15 μg), RAW264.7 (lane 3, 15 μg), U-87MG (lane 4, 15 μg), HT-1080 (lane 5, 15 μg), and U251 (lane 6, 15 μg) using TRAM2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:90000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TRAM2 Antibody (YA2825)
  • TRAM2 Antibody (YA2825) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), U2OS (lane 2, 15 μg), RAW264.7 (lane 3, 15 μg), U-87MG (lane 4, 15 μg), HT-1080 (lane 5, 15 μg), and U251 (lane 6, 15 μg) using TRAM2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:90000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TRAM2 Antibody (YA2825) Product Image WB