TRAM2 Antibody (YA2825)

Cat. No.: HY-P83080: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

TRAM2 Antibody (YA2825) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRAM2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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TRAM2 Antibody (YA2825) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

TRAM2 Antibody (YA2825) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRAM2.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 43 kDa;

Observed band size: 43 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q15035

Gene ID

7186 [NCBI]

Immunogen

A synthesized peptide derived from human TRAM2 aa250-300/370.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), U2OS (lane 2, 15 μg), RAW264.7 (lane 3, 15 μg), U-87MG (lane 4, 15 μg), HT-1080 (lane 5, 15 μg), and U251 (lane 6, 15 μg) using TRAM2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:90000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：E3 ubiquitin-protein ligase that regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis (PubMed:15121867, PubMed:18997792, PubMed:19150425, PubMed:19409903, PubMed:19815541, PubMed:9390694). Catalyzes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, IKBKE, MLST8, RIPK1 and TICAM1 (PubMed:15175328, PubMed:28489822). Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases (By similarity). Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain (PubMed:18997794). Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR (By similarity). In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE (By similarity). Acts as a regulator of mTORC1 and mTORC2 assembly by mediating 'Lys-63'-linked ubiquitination of MLST8, thereby inhibiting formation of the mTORC2 complex, while facilitating assembly of the mTORC1 complex (PubMed:28489822). Required for normal antibody isotype switching from IgM to IgG (PubMed:10514016)

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:Isomer 1 and isomer 2 are expressed in the spleen, adipose tissue, skeletal muscle, thymus, testes, heart, lungs, and brain. Isomer 2 is expressed very weakly in the heart, lungs, and brain.

Subunit：Homotrimer (By similarity). Heterotrimer with TRAF1 (PubMed:8069916).

RRID

AB_3105196

Database

Entrez Gene: 7186 Human ; 22030 Mouse ; 311786 Rat

SwissProt: Q12933 Human ; P39429 Mouse ;

OMIM: 601895 Human

Research Field

Signal Transduction

Synonyms

KIAA0057; TRAM like protein; Tram2; Translocating chain associating membrane protein 2; Translocation associated membrane protein 2

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

TRAM2 Antibody (YA2825) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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TRAM2 Antibody (YA2825)

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Background

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TRAM2 Antibody (YA2825) Related Classifications

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