PDK1 Antibody (YA5013)
(Synonyms: PDK1; PDHK1; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; mitochondrial; Pyruvate dehydrogenase kinase isoform 1)PDK1 Antibody (YA5013) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to PDK1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB; IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:20-50 |
Product Details
PDK1 Antibody (YA5013) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to PDK1.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 49 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 49 kDa
A synthesized peptide derived from human PDK1. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PDK-1 (3-Phosphoinositide-dependent protein kinase 1), a member of the protein A, G and C (AGC) family of proteins, is a Ser/Thr protein kinase. PDK-1, is the pivotal node in the PI3K pathway, has a key role in insulin and growth-factor signalling through phosphorylation and subsequent activation of a number of other AGC kinase family members, such as protein kinase B. PDK-1 is responsible for the regulation of cell proliferation and migration and it also has been found to play a key role in different cancers, pancreatic and breast cancer amongst others. Many cancer-driving mutations induce activation of PDK-1 targets including Akt, S6K (p70 ribosomal S6 kinase) and SGK. Thus, PDK1 is a critical activator of multiple pro-survival and oncogenic protein kinases, for which it has garnered considerable interest as an oncology drug target.
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Subcellular Localization
Mitochondrion matrix
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Expression
Tissue_specificity:It is primarily expressed in the heart. It is also expressed in small amounts in the liver, skeletal muscle, and pancreas.
Induction:Up-regulated via the HIF1A signaling pathway in response to hypoxia -
Isoforms & Post-Translational Modification
Q15118 has 2 isomers: Q15118-1: 49244 Da (predicted); Q15118-2: 51623 Da (predicted).
Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect (PubMed:22195962). Phosphorylated under hypoxic conditions at Thr-338 by phosphoglycerate kinase PGK1 which has an activating effect (PubMed:26942675) -
Subunit
Homodimer, and heterodimer with PDK2 (PubMed:17683942).
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SwissProt ID
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Synonyms
PDK1; PDHK1; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; mitochondrial; Pyruvate dehydrogenase kinase isoform 1
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