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Phospho-IGFBP3 (Ser183) Antibody

(Synonyms: IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3)

Phospho-IGFBP3 (Ser183) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Phospho-IGFBP3 (Ser183).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Bovine

  • Formulation:

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-IGFBP3 (Ser183) Antibody
  • Phospho-IGFBP3 (Ser183) Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-2000 1:100-200

Product Details

Description

Phospho-IGFBP3 (Ser183) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Phospho-IGFBP3 (Ser183).

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Bovine
  • Observed Molecular Weight
    Observed band size: 32 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 31 kDa
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding S183 of human IGFBP3 protein.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-IGFBP3 (Ser183) Antibody
    Western blot analysis was performed on protein extracts (35 μg) from U87 (lane 2) and A549 (lane 3) using Phospho-IGFBP3 (Ser183) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% Bovine Serum Albumin (BSA, HY-D0842) in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% BSA in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% BSA in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    IGFBP3 multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity. Promotes testicular germ cell apoptosis. Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1. Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade. In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis[1][2][3][4][5][6][7].

  • Subcellular Localization

    Secreted,Nucleus

  • Expression


    Tissue_Specificity: Expressed by most tissues. Present in plasma
    Induction: Up-regulated in the presence of IGF1, insulin and other growth-stimulating factors such as growth hormone, EGF and phorbol esters

  • Isoforms & Post-Translational Modification

    P17936 has two isomers: P17936-1: 31674 Da (predicted); P17936-2: 32223 Da (predicted).
    Phosphorylated by FAM20C in the extracellular medium

  • Subunit

    Interacts with XLKD1 (By similarity)

  • SwissProt ID

    P17936

  • Gene ID
    3486 [NCBI]
  • Synonyms

    IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3

Phospho-IGFBP3 (Ser183) Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (35 μg) from U87 (lane 2) and A549 (lane 3) using Phospho-IGFBP3 (Ser183) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% Bovine Serum Albumin (BSA, HY-D0842) in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% BSA in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% BSA in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Phospho-IGFBP3 (Ser183) Antibody Product Image
    1/16
  • Experimental Validation Results for Phospho-IGFBP3 (Ser183) Antibody
  • Phospho-IGFBP3 (Ser183) Antibody Product Image
  • Experimental Validation Results for Phospho-IGFBP3 (Ser183) Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (35 μg) from U87 (lane 2) and A549 (lane 3) using Phospho-IGFBP3 (Ser183) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% Bovine Serum Albumin (BSA, HY-D0842) in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% BSA in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% BSA in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Phospho-IGFBP3 (Ser183) Antibody
  • Phospho-IGFBP3 (Ser183) Antibody Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (35 μg) from U87 (lane 2) and A549 (lane 3) using Phospho-IGFBP3 (Ser183) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% Bovine Serum Albumin (BSA, HY-D0842) in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% BSA in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% BSA in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Phospho-IGFBP3 (Ser183) Antibody Product Image WB