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CD73 Antibody (YA528)

Customer Review

Based on 1 Customer Validation

CD73 Antibody (YA528) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD73.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD73 Antibody (YA528)
  • CD73 Antibody (YA528) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-1:5000 1:50-1:200

Product Details

Description

CD73 Antibody (YA528) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD73.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 63 kDa
Species Reactivity Database

Entrez Gene: 4907 Human

SwissProt: P21589 Human

Immunogen

Synthetic peptide corresponding to Human CD73.AA range:491-536.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102110

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD73 Antibody (YA528)
    Western blot analysis of extracts from HT-29 (lane 2(20μg) and HT-29 (lane 3(40μg)using CD73 (HY-P80387) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80387, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084)

  • Subcellular Localization

    Cell membrane; Lipid-anchor, GPI-anchor

  • Isoforms & Post-Translational Modification

    P21589 has 2 isomers: P21589-1: 63368 Da (predicted); P21589-2: 57949 Da (predicted).

  • Subunit

    Homodimer

  • SwissProt ID

    P21589

  • Gene ID
    4907 [NCBI]
  • Research Field

    Cardiovascular

CD73 Antibody (YA528) Related Classifications

100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    CD73 Antibody (YA528) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Western blot analysis of extracts from HT-29 (lane 2(20μg) and HT-29 (lane 3(40μg)using CD73 (HY-P80387) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80387, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD73 Antibody (YA528) Product Image
    1/16
  • Experimental Validation Results for CD73 Antibody (YA528)
  • CD73 Antibody (YA528) Product Image
  • Experimental Validation Results for CD73 Antibody (YA528)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HT-29 (lane 2(20μg) and HT-29 (lane 3(40μg)using CD73 (HY-P80387) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80387, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD73 Antibody (YA528)
  • CD73 Antibody (YA528) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HT-29 (lane 2(20μg) and HT-29 (lane 3(40μg)using CD73 (HY-P80387) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80387, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD73 Antibody (YA528) Product Image WB