NT5E - 5'-nucleotidase ecto Gene

Also Known as NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 4907

About NT5E

Cytogenetic location: 6q14.3 Genomic coordinates (GRCh38): 6:85,450,083-85,495,784 (from NCBI)

This gene has 5 transcripts (splice variants), 249 orthologues and is associated with 2 phenotypes. Ubiquitous expression in endometrium (RPKM 30.0), ovary (RPKM 19.6) and 22 other tissues.

Summary

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

NT5E Products (2)

mRNA	Protein	Name
NM_002526.4	NP_002517.1	5'-nucleotidase isoform 1 preproprotein
NM_001204813.2	NP_001191742.1	5'-nucleotidase isoform 2 preproprotein

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables 5'-deoxynucleotidase activity	IDA IDA: Inferred from direct assay	22997138	GOA
enables 5'-nucleotidase activity	IDA IDA: Inferred from direct assay	7595232	GOA
enables 5'-nucleotidase activity	IMP IMP: Inferred from mutant phenotype	24887587	GOA
enables GMP 5'-nucleotidase activity	IDA IDA: Inferred from direct assay	22997138	GOA
enables IMP 5'-nucleotidase activity	IDA IDA: Inferred from direct assay	22997138	GOA
enables identical protein binding	IDA IDA: Inferred from direct assay	22997138	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	32296183	GOA
enables thymidylate 5'-phosphatase activity	IDA IDA: Inferred from direct assay	22997138	GOA
enables zinc ion binding	IDA IDA: Inferred from direct assay	22997138	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in ATP metabolic process	IDA IDA: Inferred from direct assay	25644539	GOA
involved in leukocyte cell-cell adhesion	IDA IDA: Inferred from direct assay	7595232	GOA
involved in response to ATP	IDA IDA: Inferred from direct assay	25644539	GOA

Cellular Component GO Annotation	Evidence	References	Source
located in membrane	IDA IDA: Inferred from direct assay	2129526	GOA
is active in plasma membrane	IDA IDA: Inferred from direct assay	25644539	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

NT5E Protein Structure

Metallophos

5_nucleotid_C

0
100
200
300
400
500
574 a.a.

Protein Preferred Names	Protein Names
5'-nucleotidase	5'-NT Purine 5-Prime-Nucleotidase ecto-5'-nucleotidase

NT5E Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	NT5E	P21589	RNF24	Homo sapiens	Q9Y225-2	Validated Y2H	32296183
Intra	NT5E	P21589	RNF24	Homo sapiens	Q9Y225-2	Y2H Prey Pooling	32296183
Intra	NT5E	P21589	RNF24	Homo sapiens	Q9Y225-2	Y2H Array	32296183
Intra	NT5E	P21589	ZNRF4	Homo sapiens	Q8WWF5	Y2H Prey Pooling	32296183
Intra	NT5E	P21589	ZNRF4	Homo sapiens	Q8WWF5	Y2H Array	32296183
Intra	NT5E	P21589	ZNRF4	Homo sapiens	Q8WWF5	Validated Y2H	32296183

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant NT5E Proteins

Cat. No.	Product Name	Accession	Purity
HY-P70010	CD73/5'-Nucleotidase Protein, Human (HEK293, His)	P21589-1 (W27-K547)	≥ 95%, as determined by reducing SDS-PAGE.
HY-P70492	CD73/5'-Nucleotidase Protein, Human (T376A, M379T, HEK293, His)	P21589-1 (W27-K547, T376A, M379T)	≥ 95%, as determined by reducing SDS-PAGE.
HY-P74272	CD73/5'-Nucleotidase Protein, Human (T376A, HEK293, Fc)	P21589-1/NP_002517.1 (W27-K547, T376A)	≥ 90%, as determined by reducing SDS-PAGE.
HY-P77534	CD73/5'-Nucleotidase Protein, Human (Biotinylated, HEK293, His-Avi)	P21589-1/NP_002517.1 (W27-K547, T376A)	≥ 85%, as determined by reducing SDS-PAGE.

NT5E Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P80387	CD73 Antibody (YA528)	WB, IHC-P	Human
HY-P80388	CD73 Antibody (YA797)	WB, IHC-P, ICC/IF, FC	Human
HY-P83708M	Human CD73 Antibody (PE) (YA3515)	FC	Human
HY-P84179	CD73 Antibody (YA3876)	WB, IHC-P, FC, ELISA	Human, Mouse, Rat, Rabbit
HY-P84179A	CD73 Antibody (YA3876)(PBS only)	WB, IHC-P, FC, ELISA	Human, Mouse, Rat, Rabbit
HY-P85093	CD73 Antibody (YA4785)	IHC-P, ICC/IF, ELISA	Human

Related Diseases

Diseases	Alias
Spondylometaphyseal Dysplasia With Corneal Dystrophy	SMDCD Spondylometaphyseal Dysplasia-Corneal Dystrophy Syndrome Smd-Corneal Dystrophy Syndrome
Arterial Calcification Of Infancy	Idiopathic Infantile Arterial Calcification Generalized Arterial Calcification Of Infancy Iiac Occlusive Infantile Arteriopathy Infantile Arteriosclerosis Gaci Idiopathic Obliterative Arteriopathy Generalized Arterial Calcification In Infancy Arteriopathia Calcificans Infantum Diffuse Arterial Calcifying Elastopathy Of Infancy Infantile Calcifying Arteriopathy Medial Coronary Sclerosis Of Infancy Coronary Sclerosis, Medial, Of Infancy
Brittle Bone Disorder	Osteogenesis Imperfecta Brittle Bone Disease Fragilitas Ossium Osteopsathyrosis Lobstein Disease Oi Vrolik Disease Lobstein'S Disease Lobstein'S Syndrome Vrolik'S Disease Porak And Durante Disease Glass Bone Disease Osteogenesis Imperfecta, Dominant Perinatal Lethal Osteogenesis Imperfecta, Recessive Perinatal Lethal Brittle Bone Syndrome Oi - [Osteogenesis Imperfecta] Ossium Fragility Osteitis Fragilitans Bony Fragility Blue Sclera With Fragility Of Bone And Deafness White Blue Sclera - Fragility Of Bone - Deafness
Osteochondrodysplasia	Chondrodystrophy Skeletal Dysplasia Congenital Anomaly Of Cartilage Osteochondrodysplasias Cartilage Development Disorder Osteochondrodysplasia Syndrome Dysplasia, Skeletal Mucopolysaccharidosis Iv
Retinitis Pigmentosa	RP Rod-Cone Dystrophy Autosomal Recessive Retinitis Pigmentosa Non-Syndromic Retinitis Pigmentosa Pericentral Pigmentary Retinopathy Pigmentary Retinopathy Tapetoretinal Degeneration Rcd Retinitis Pigmentosa Autosomal Recessive ARRP Retinitis Pigmentosa, Autosomal Recessive Retinitis Pigmentosa 1
Brain Edema	Intracranial Swelling Wet Brain Cerebral Edema
Pseudoxanthoma Elasticum	PXE Gronblad-Strandberg Syndrome Pseudoxanthoma Elasticum, Modifier Of Severity Of Gronblad-Strandberg-Touraine Syndrome Gronblad Strandberg Syndrome Groenblad-Strandberg Syndrome Nevus Elasticus Pxe - [Pseudoxanthoma Elasticum]
Anal Fistula	Anorectal Fistula Rectal Fistula Allingham Ulcer Perianal Fistula Fistula In Ano Anorectum Fistula
Nervous System Disease	Abnormality Of The Nervous System Nervous System Diseases Nervous System Disorder
Calciphylaxis	Idiopathic Calciphylaxis
Inflammatory Bowel Disease 14	IBD14 Inflammatory Bowel Disease 14, Susceptibility To Bowel Disease, Inflammatory, Type 14
Tibial Nerve Palsy
Germ Cell And Embryonal Cancer	Germ Cell And Embryonal Neoplasm
Intermittent Claudication	Charcot'S Syndrome Amyotrophic Lateral Sclerosis
Immune Deficiency Disease	Immunodeficiency Primary Immunodeficiency Disease Primary Immunodeficiency Immunologic Deficiency Syndromes Hypoimmunity Immune Deficiency Disorder Immunodeficiency Syndrome Immune Disorder Primary Immune Deficiency Disorder Immune System Diseases Human Immunodeficiency Virus Infection Hiv - [Human Immunodeficiency Virus Infection] Hiv Positive Nos Hiv Disease Acquired Immune Deficiency Syndrome-Related Complex Aids-Like Syndrome Aids-Related Complex Nos Arc - [Aids-Related Complex] Immunodeficiency Due To Human Immunodeficiency Virus Infection Unspecified Human Immunodeficiency Virus Disease Hiv Disease Nos Human Immunodeficiency Virus Positive Nos Hiv Nos Deficiency Of Complement Initial Pathway Deficiency Of Complement Terminal Pathway Cfdd - [Complement Factor D Deficiency] Immunodeficiency With Nk-Cell - [Natural-Killer Cell] Deficiency Nonfamilial Hypogammaglobulinaemia Common Variable Immune Deficiency Nonfamilial Agammaglobulinaemia Common Variable Agammaglobulinaemia Agammaglobulinaemia Nos Agammaglobulinaemia Antibody Deficiency Syndrome Hypogammaglobulinaemia Antibody Deficiency Syndrome Acquired Agammaglobulinaemia Nos Hypogammaglobulinaemia Nos Hyper Igm
Limb Ischemia
Dermatitis, Atopic, 7	ATOD7 Dermatitis, Atopic, Susceptibility To, 7 Atopic Dermatitis 7 Dermatitis, Atopic 7
Retinitis Pigmentosa 89	RP89
Dental Pulp Necrosis	Pulp Necrosis Necrotic Pulp Necrosis Of The Pulp Dead Pulp Devitalised Pulp Putrescent Pulpitis Devitalized Tooth Dental Pulp Gangrene Pulp Gangrene Nonvital Tooth Pulpless Tooth Putrescent Pulp
Periarthritis
Calcification Of Joints And Arteries	Hereditary Arterial And Articular Multiple Calcification Syndrome CALJA Arterial Calcification Due To Deficiency Of Cd73 Acdc Arterial Calcification And Distal Joint Calcification Arterial Calcification Due To Cd73 Deficiency Arterial Calcification Due To Deficiency Of Cd73:Acdc Calcification Of Joints And Arteries Calja
Dental Pulp Disease	Dental Pulp Diseases Dental Pulp Disorder Disorder Of Pulp Of Tooth Pulp Disorder
T-Cell Acute Lymphoblastic Leukemia	T-Cell Leukemia Acute T Cell Leukemia Precursor T Lymphoblastic Leukemia Precursor T-Lymphoblastic Lymphoma/Leukemia T Acute Lymphoblastic Leukemia T-Cell Acute Lymphocytic Leukaemia T-Cell Lymphoblastic Leukemia/Lymphoma Leukemia T-Cell Leukemia, T-Cell Leukemia, Acute, Lymphoblastic, T-Cell Leukemia, T-Cell Acute Lymphoblastic Leukemia, Acute T-Cell Precursor T-Cell Lymphoblastic Leukemia-Lymphoma Precursor T-Cell Lymphoblastic Lymphoma Precursor T Cell Lymphoblastic Leukemia/Lymphoblastic Lymphoma
Adhesions Of Uterus	Intrauterine Adhesions Intrauterine Synechiae Band Of Uterus Asherman Syndrome Internal Adhesion Of Uterus Internal Band Of Uterus

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Clinical Phase
Pre-clinical Phase

Clinical Phase

Inhibitory Antibodies (5)

Cat. No.	Product Name	Effect	Purity	Phase
HY-P991009	Mavrostobart		98.50%	Phase 2
HY-P991332	JAB-BX102	Inhibitor	/	Phase 2
HY-P991330	BMS-986179	Inhibitor	/	Phase 2
HY-P990771	Uprevstobart		96.98%	Phase 1
HY-P991443	IPH5301	Inhibitor	/	Phase 1

Pre-clinical Phase

Bioactive Molecules (3)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS09612	NT5E Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS09613	Nt5e Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS09614	Nt5e Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Inhibitory Antibodies (2)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-P990900	Ansipastobart		96.259%	Unkown
HY-P991331	BC-010	Inhibitor	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Rattus norvegicus	NT5E	RGD	RGD:61956
Macaca mulatta	NT5E	VGNC	VGNC:75537
Canis familiaris	NT5E	VGNC	VGNC:44000
Felis catus	NT5E	VGNC	VGNC:68574
Mus musculus	NT5E	MGD	MGI:99782
Others	NT5E	NCBI