Copy Link Print Information

CD73 Antibody (YA797)

Customer Review

Based on 1 Customer Validation

CD73 Antibody (YA797) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD73.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD73 Antibody (YA797)
  • CD73 Antibody (YA797) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
You May Still Need
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500 1:200-1:600 1:100 1:100

Product Details

Description

CD73 Antibody (YA797) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD73.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 63 kDa
Species Reactivity Database

Entrez Gene: 4907 Human

SwissProt: P21589 Human

Immunogen

Synthetic peptide corresponding to Human CD73 27-549.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3102474

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD73 Antibody (YA797)
    Western blot analysis of extracts from U87(lane 2(20μg) or lane 3(40μg)), using CD73 (HY-P80388) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80388, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084)

  • Subcellular Localization

    Cell membrane; Lipid-anchor, GPI-anchor

  • Isoforms & Post-Translational Modification

    P21589 has 2 isomers: P21589-1: 63368 Da (predicted); P21589-2: 57949 Da (predicted).

  • Subunit

    Homodimer

  • SwissProt ID

    P21589

  • Gene ID
    4907 [NCBI]
  • Research Field

    Cardiovascular

CD73 Antibody (YA797) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from U87(lane 2(20μg) or lane 3(40μg)), using CD73 (HY-P80388) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80388, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD73 Antibody (YA797) Product Image
    1/16
  • Experimental Validation Results for CD73 Antibody (YA797)
  • CD73 Antibody (YA797) Product Image
  • Experimental Validation Results for CD73 Antibody (YA797)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from U87(lane 2(20μg) or lane 3(40μg)), using CD73 (HY-P80388) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80388, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD73 Antibody (YA797)
  • CD73 Antibody (YA797) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from U87(lane 2(20μg) or lane 3(40μg)), using CD73 (HY-P80388) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80388, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD73 Antibody (YA797) Product Image WB