Human CD73 Antibody (PE) (YA3515)
(Synonyms: NT5E; NT5; NTE; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; CD73)Human CD73 Antibody (PE) (YA3515) is a mouse-derived and phycoerythrin (PE) IgG1 monoclonal antibody, targeting to CD73.
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Host:
Mouse
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Isotype:
IgG
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Application:
FC
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% Proclin300, 1% BSA.
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Conjugation:
PE
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
FC
FC: Flow Cytometry
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|---|---|
| Dilution Ratio | 5μL per test for million cells or whole blood in 100 μL volume. |
Product Details
Human CD73 Antibody (PE) (YA3515) is a mouse-derived and phycoerythrin (PE) IgG1 monoclonal antibody, targeting to CD73.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 70 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 63 kDa
A synthesized peptide derived from Human CD73
Affinity
PE
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% Proclin300, 1% BSA.
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Storage & Stability
Stored at 2-8°C for 1 year, protect from light, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
CD73 (NT5E) is a GPI-anchored extracellular 5'-nucleotidase that regulates immune tolerance, inflammation, and tumor immune escape by converting AMP into the immunosuppressive molecule adenosine. In immunological assays, CD73 serves as a key marker for studying Treg function, the immunosuppressive microenvironment, and tumor immunity.
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Subcellular Localization
Cell membrane; Lipid-anchor, GPI-anchor
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Isoforms & Post-Translational Modification
CD73 has 2 isomers: P21589-1: 63368 Da (predicted); P21589-2: 57949 Da (predicted).
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Subunit
Homodimer
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SwissProt ID
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Synonyms
NT5E; NT5; NTE; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; CD73
Documentation
[1]. Garavaglia S, et al. The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism. Biochem J. 2012 Jan 1;441(1):131-41. [Content Brief]
[2]. Heuts DP, et al. Crystal structure of a soluble form of human CD73 with ecto-5'-nucleotidase activity. Chembiochem. 2012 Nov 5;13(16):2384-91. [Content Brief]
[3]. Knapp K, et al. Crystal structure of the human ecto-5'-nucleotidase (CD73): insights into the regulation of purinergic signaling. Structure. 2012 Dec 5;20(12):2161-73. [Content Brief]
[4]. Fausther M, et al. NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein. PLoS One. 2014;9(6):e98568. [Content Brief]
[5]. Scaletti E, et al. Substrate binding modes of purine and pyrimidine nucleotides to human ecto-5'-nucleotidase (CD73) and inhibition by their bisphosphonic acid derivatives. Purinergic Signal. 2021 Dec;17(4):693-704. [Content Brief]
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