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CD73 Antibody (YA3876)

(Synonyms: NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA)
Customer Review

Based on 1 Customer Validation

CD73 Antibody (YA3876) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD73.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat, Rabbit

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • CD73 Antibody (YA3876) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

CD73 Antibody (YA3876) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD73.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Rabbit
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 63 kDa
Immunogen

Purified recombinant fragment of human NT5E (AA: 30-250) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD73 Antibody (YA3876)
    Western blot analysis of extracts from HT-29 (lane2, 20μg), U87 (lane3, 20μg), HepG2 (lane4, 20μg) and Jurkat (lane5, 20μg) using CD73 Antibody (HY-P84179). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    Immunohistochemical analysis of paraffin-embedded human ‌Colorectal cancer tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    Immunohistochemical analysis of paraffin-embedded human ‌solitary fibrous tumor tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    Immunohistochemical analysis of paraffin-embedded human ‌cervix of uterus tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    Immunohistochemical analysis of paraffin-embedded human ‌uterine appendages tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    Immunohistochemical analysis of paraffin-embedded human ‌esophagus tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    Immunohistochemical analysis of paraffin-embedded human ‌lungs tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    Immunohistochemical analysis of paraffin-embedded human ‌Liver tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    Immunohistochemical analysis of paraffin-embedded human ‌placenta tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    CD73 (NT5E) is a GPI-anchored extracellular 5'-nucleotidase that regulates immune tolerance, inflammation, and tumor immune escape by converting AMP into the immunosuppressive molecule adenosine. In immunological assays, CD73 serves as a key marker for studying Treg function, the immunosuppressive microenvironment, and tumor immunity.

  • Subcellular Localization

    Cell membrane; Lipid-anchor, GPI-anchor

  • Isoforms & Post-Translational Modification

    CD73 has 2 isomers: P21589-1: 63368 Da (predicted); P21589-2: 57949 Da (predicted).

  • Subunit

    Homodimer

  • SwissProt ID

    P21589

  • Gene ID
    4907 [NCBI]
  • Synonyms

    NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA

CD73 Antibody (YA3876) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HT-29 (lane2, 20μg), U87 (lane3, 20μg), HepG2 (lane4, 20μg) and Jurkat (lane5, 20μg) using CD73 Antibody (HY-P84179). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Colorectal cancer tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌solitary fibrous tumor tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌cervix of uterus tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌uterine appendages tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌esophagus tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌lungs tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Liver tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌placenta tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD73 Antibody (YA3876) Product Image
    1/16
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • CD73 Antibody (YA3876) Product Image
  • Experimental Validation Results for CD73 Antibody (YA3876)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HT-29 (lane2, 20μg), U87 (lane3, 20μg), HepG2 (lane4, 20μg) and Jurkat (lane5, 20μg) using CD73 Antibody (HY-P84179). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Colorectal cancer tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌solitary fibrous tumor tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌cervix of uterus tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌uterine appendages tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌esophagus tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌lungs tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Liver tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌placenta tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • Experimental Validation Results for CD73 Antibody (YA3876)
  • CD73 Antibody (YA3876) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HT-29 (lane2, 20μg), U87 (lane3, 20μg), HepG2 (lane4, 20μg) and Jurkat (lane5, 20μg) using CD73 Antibody (HY-P84179). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • CD73 Antibody (YA3876) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Colorectal cancer tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD73 Antibody (YA3876) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌solitary fibrous tumor tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD73 Antibody (YA3876) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌cervix of uterus tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD73 Antibody (YA3876) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌uterine appendages tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD73 Antibody (YA3876) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌esophagus tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD73 Antibody (YA3876) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌lungs tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD73 Antibody (YA3876) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Liver tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD73 Antibody (YA3876) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌placenta tissue using CD73 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84179, 1:1000 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD73 Antibody (YA3876) Product Image WB
  • CD73 Antibody (YA3876) Product Image IHC-P
  • CD73 Antibody (YA3876) Product Image IHC-P
  • CD73 Antibody (YA3876) Product Image IHC-P
  • CD73 Antibody (YA3876) Product Image IHC-P
  • CD73 Antibody (YA3876) Product Image IHC-P
  • CD73 Antibody (YA3876) Product Image IHC-P
  • CD73 Antibody (YA3876) Product Image IHC-P
  • CD73 Antibody (YA3876) Product Image IHC-P