C5b-9 Antibody (YA7712)
C5b-9 Antibody (YA7712) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to C5b-9.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, IF-Tissue, IP
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Reactivity :
Human
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:50-200 | 1:50-200 | 1:50-200 | 1:20-50 |
Product Details
C5b-9 Antibody (YA7712) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to C5b-9.
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Host Rabbit
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Clonality Recombinant
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 70 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 63 kDa
A synthesized peptide derived from human Complement C9
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
C5b-9 is a Pore-forming component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Constitutes the pore-forming subunit of the MAC complex: during MAC assembly, C9 associates with the C5b8 intermediate complex, and polymerizes to complete the pore[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Secreted,Target cell membrane
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Expression
Tissue_Specificity: Plasma (at protein level) -
Isoforms & Post-Translational Modification
P02748: 559 amino acids, molecular weight 63173 Da.
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Subunit
Homooligomer; about 20 C9 chains oligomerize to give rise to a huge beta-barrel that forms a 100 Angstrom diameter pore in target membranes (PubMed:26841934, PubMed:30111885, PubMed:34752492)
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SwissProt ID
Documentation
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