PRMT6 Antibody (YA5188)

Cat. No.: HY-P85496: Data Sheet User Guide for Antibodies
FAQs
Technical Support

PRMT6 Antibody (YA5188) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PRMT6.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PRMT6 Antibody (YA5188) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PRMT6.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q96LA8

Gene ID

55170 [NCBI]

Immunogen

Purified recombinant human PRMT6 protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:300

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA (PubMed:17898714, PubMed:18077460, PubMed:18079182, PubMed:19405910, PubMed:30420520). Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates (PubMed:17898714, PubMed:18077460, PubMed:18079182, PubMed:19405910). Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a (PubMed:17898714, PubMed:18077460, PubMed:18079182). H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3) (PubMed:17898714, PubMed:18077460). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53 (PubMed:19509293). Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity (PubMed:16600869). Methylates HMGA1 (PubMed:16157300, PubMed:16159886). Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator (By similarity). May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC) (PubMed:17267505). Methylates GPS2, protecting GPS2 from ubiquitination and degradation (By similarity). Methylates SIRT7, inhibiting SIRT7 histone deacetylase activity and promoting mitochondria biogenesis (PubMed:30420520)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:It is highly expressed in the kidneys and testes.

Isoforms & Post-Translational Modification：Q96LA8 has 2 isomers: Q96LA8-1: 41938 Da (predicted); Q96LA8-2: 32509 Da (predicted).
Automethylation enhances its stability and antiretroviral activity

Subunit：Interacts with EPB41L3 and NCOA1

Synonyms

ANM6_HUMAN; Chromobox protein homolog 7; FLJ10559; FLJ51477; Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; Histone-arginine N-methyltransferase PRMT6; HMT1 hnRNP methyltransferase like 6.; HRMT1L6; OTTHUMP00000012633; PRMT 6; prmt6; Protein arginine methyltransferase 6; Protein arginine N methyltransferase 6; Protein arginine N-methyltransferase 6.

Documentation

User Guide for Antibodies (1077 KB)