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SPINT1 Antibody (YA7833)

(Synonyms: HAI; HAI1; MANSC2)
Customer Review

Based on 1 Customer Validation

SPINT1 Antibody (YA7833) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SPINT1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human, Dog, Rat, Monkey, Mouse

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
  • SPINT1 Antibody (YA7833) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:200 1:100-250 1:100

Product Details

Description

SPINT1 Antibody (YA7833) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SPINT1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Dog, Rat, Monkey, Mouse
  • Observed Molecular Weight
    Observed band size: 58 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 58 kDa
Immunogen

Full length human recombinant protein of human SPINT1 produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SPINT1 Antibody (YA7833)
    Western blot analysis of extracts from HCT116 (lane2(20μg), HepG2 (lane3(20μg), MCF-7 (lane4(20μg) and Hela (lane5(20μg) using SPINT1 Antibody (HY-P88149). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
    Immunocytochemistry analysis of PC-3 cells labeling SPINT1 with SPINT1 Antibody (HY-P88149) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SPINT1 Antibody (HY-P88149) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
    Immunocytochemistry analysis of PC-3 cells labeling SPINT1 with SPINT1 Antibody (HY-P88149) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SPINT1 Antibody (HY-P88149) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    SPINT1 is an Inhibitor of HGFAC. Inhibits serine protease activity of ST14/matriptase in vitro. Inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain[1][2][3].

  • Subcellular Localization

    Secreted,Cytoplasm,Cell membrane

  • Isoforms & Post-Translational Modification

    O43278 has two isomers: O43278-1: 58398 Da (predicted); O43278-2: 56885 Da (predicted).

  • Subunit

    Interacts with HGFAC (PubMed:15713485)

  • SwissProt ID

    O43278

  • Synonyms

    HAI; HAI1; MANSC2

SPINT1 Antibody (YA7833) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HCT116 (lane2(20μg), HepG2 (lane3(20μg), MCF-7 (lane4(20μg) and Hela (lane5(20μg) using SPINT1 Antibody (HY-P88149). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling SPINT1 with SPINT1 Antibody (HY-P88149) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SPINT1 Antibody (HY-P88149) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling SPINT1 with SPINT1 Antibody (HY-P88149) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SPINT1 Antibody (HY-P88149) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SPINT1 Antibody (YA7833) Product Image
    1/16
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
  • SPINT1 Antibody (YA7833) Product Image
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HCT116 (lane2(20μg), HepG2 (lane3(20μg), MCF-7 (lane4(20μg) and Hela (lane5(20μg) using SPINT1 Antibody (HY-P88149). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling SPINT1 with SPINT1 Antibody (HY-P88149) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SPINT1 Antibody (HY-P88149) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling SPINT1 with SPINT1 Antibody (HY-P88149) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SPINT1 Antibody (HY-P88149) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
  • Experimental Validation Results for SPINT1 Antibody (YA7833)
  • SPINT1 Antibody (YA7833) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HCT116 (lane2(20μg), HepG2 (lane3(20μg), MCF-7 (lane4(20μg) and Hela (lane5(20μg) using SPINT1 Antibody (HY-P88149). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • SPINT1 Antibody (YA7833) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling SPINT1 with SPINT1 Antibody (HY-P88149) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SPINT1 Antibody (HY-P88149) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SPINT1 Antibody (YA7833) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling SPINT1 with SPINT1 Antibody (HY-P88149) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SPINT1 Antibody (HY-P88149) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SPINT1 Antibody (YA7833) Product Image WB
  • SPINT1 Antibody (YA7833) Product Image ICC
  • SPINT1 Antibody (YA7833) Product Image ICC