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LGI1 Antibody (YA2885)

(Synonyms: ADLTE; ADPAEF; ADPEAF; Epitempin 1; EPITEMPIN; EPT; ETL1; LGI1)
Customer Review

Based on 1 Customer Validation

LGI1 Antibody (YA2885) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LGI1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for LGI1 Antibody (YA2885)
  • Experimental Validation Results for LGI1 Antibody (YA2885)
  • Experimental Validation Results for LGI1 Antibody (YA2885)
  • LGI1 Antibody (YA2885) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50

Product Details

Description

LGI1 Antibody (YA2885) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LGI1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 64 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 64 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Lgi1 aa150-250.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105255

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LGI1 Antibody (YA2885)
    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using LGI1 Antibody (HY-P83140) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for LGI1 Antibody (YA2885)
    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using LGI1 Antibody (YA2885). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83140, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for LGI1 Antibody (YA2885)
    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using LGI1 Antibody (YA2885). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83140, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    LGI1 regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors. Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival

  • Subcellular Localization

    Secreted; Synapse; Cytoplasm; Golgi apparatus; Secreted; Cytoplasm; Endoplasmic reticulum; Cytoplasm

  • Expression


    Tissue_specificity:Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas; Expressed in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is between 3 and 5 times more abundant than in the hippocampus (at protein level) (PubMed:16787412) . Expression is absent in the cerebellum (PubMed:16787412) ; Abundantly expressed in the occipital cortex and weakly expressed in the hippocampus (at protein level)

    Induction:Down-regulated in neuroblastoma cells

  • Isoforms & Post-Translational Modification

    O95970 has 3 isomers: O95970-1: 63818 Da (predicted); O95970-2: 33121 Da (predicted); O95970-3: 58302 Da (predicted).
    Glycosylated

  • Subunit

    Oligomer. Interacts with KCNA1 within a complex containing KCNA1, KCNA4 and KCNAB1. Part of a complex containing ADAM22, DLG4/PSD95 and CACNG2/Stargazin (PubMed:27066583). Can bind to ADAM11 and ADAM23

  • SwissProt ID

    O95970

  • Gene ID
    9211 [NCBI]
  • Synonyms

    ADLTE; ADPAEF; ADPEAF; Epitempin 1; EPITEMPIN; EPT; ETL1; LGI1

  • Research Field

    Neuroscience

LGI1 Antibody (YA2885) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using LGI1 Antibody (HY-P83140) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using LGI1 Antibody (YA2885). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83140, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using LGI1 Antibody (YA2885). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83140, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • LGI1 Antibody (YA2885) Product Image
    1/16
  • Experimental Validation Results for LGI1 Antibody (YA2885)
  • Experimental Validation Results for LGI1 Antibody (YA2885)
  • Experimental Validation Results for LGI1 Antibody (YA2885)
  • LGI1 Antibody (YA2885) Product Image
  • Experimental Validation Results for LGI1 Antibody (YA2885)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using LGI1 Antibody (HY-P83140) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for LGI1 Antibody (YA2885)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using LGI1 Antibody (YA2885). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83140, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for LGI1 Antibody (YA2885)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using LGI1 Antibody (YA2885). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83140, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for LGI1 Antibody (YA2885)
  • Experimental Validation Results for LGI1 Antibody (YA2885)
  • Experimental Validation Results for LGI1 Antibody (YA2885)
  • LGI1 Antibody (YA2885) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using LGI1 Antibody (HY-P83140) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • LGI1 Antibody (YA2885) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using LGI1 Antibody (YA2885). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83140, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • LGI1 Antibody (YA2885) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using LGI1 Antibody (YA2885). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83140, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • LGI1 Antibody (YA2885) Product Image WB
  • LGI1 Antibody (YA2885) Product Image IHC-P
  • LGI1 Antibody (YA2885) Product Image IHC-P