PPM1D Antibody (YA7796)

Cat. No.: HY-P88112: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

PPM1D Antibody (YA7796) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PPM1D.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

PPM1D Antibody (YA7796) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PPM1D.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 66.5 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O15297

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-375 of human PPM1D produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane2(20μg), MCF-7 (lane3(20μg), A549 (lane4(20μg) and Jurkat (lane5(20μg) using PPM1D Antibody (HY-P88112). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Involved in the negative regulation of p53 expression (PubMed:23242139). Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins (PubMed:15870257, PubMed:16311512). Mediates MAPK14 dephosphorylation and inactivation (PubMed:21283629). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (By similarity)

Subcellular Localization：Nucleus,Cytoplasm, cytosol

Expression：
Tissue_Specificity: Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts
Induction: By p53/TP53

Isoforms & Post-Translational Modification：O15297 has two isomers: O15297-1: 66675 Da (predicted); O15297-2: 47211 Da (predicted).

Subunit：Interacts with CHEK1 and CHEK2; dephosphorylates them

Synonyms

PP2C-DELTA; WIP1

Documentation

Select Batch:

Data Sheet (230 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)