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PPM1D Antibody (YA7796)

(Synonyms: PP2C-DELTA; WIP1)
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Based on 1 Customer Validation

PPM1D Antibody (YA7796) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PPM1D.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PPM1D Antibody (YA7796)
  • PPM1D Antibody (YA7796) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-2000

Product Details

Description

PPM1D Antibody (YA7796) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PPM1D.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 66.5 kDa
Immunogen

Human recombinant protein fragment corresponding to amino acids 1-375 of human PPM1D produced in E.coli.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PPM1D Antibody (YA7796)
    Western blot analysis of extracts from Hela (lane2(20μg), MCF-7 (lane3(20μg), A549 (lane4(20μg) and Jurkat (lane5(20μg) using PPM1D Antibody (HY-P88112). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PPM1D is involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation. Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity[1][2][3][4].

  • Subcellular Localization

    Nucleus,Cytoplasm, cytosol

  • Expression


    Tissue_Specificity: Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts
    Induction: By p53/TP53

  • Isoforms & Post-Translational Modification

    O15297 has two isomers: O15297-1: 66675 Da (predicted); O15297-2: 47211 Da (predicted).

  • Subunit

    Interacts with CHEK1 and CHEK2; dephosphorylates them

  • SwissProt ID

    O15297

  • Synonyms

    PP2C-DELTA; WIP1

PPM1D Antibody (YA7796) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), MCF-7 (lane3(20μg), A549 (lane4(20μg) and Jurkat (lane5(20μg) using PPM1D Antibody (HY-P88112). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • PPM1D Antibody (YA7796) Product Image
    1/16
  • Experimental Validation Results for PPM1D Antibody (YA7796)
  • PPM1D Antibody (YA7796) Product Image
  • Experimental Validation Results for PPM1D Antibody (YA7796)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), MCF-7 (lane3(20μg), A549 (lane4(20μg) and Jurkat (lane5(20μg) using PPM1D Antibody (HY-P88112). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PPM1D Antibody (YA7796)
  • PPM1D Antibody (YA7796) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2(20μg), MCF-7 (lane3(20μg), A549 (lane4(20μg) and Jurkat (lane5(20μg) using PPM1D Antibody (HY-P88112). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • PPM1D Antibody (YA7796) Product Image WB