ERCC1 Antibody (YA5357)

Cat. No.: HY-P85665: Ficha de datos User Guide for Antibodies
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ERCC1 Antibody (YA5357) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to ERCC1.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

ERCC1 Antibody (YA5357) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to ERCC1.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Observed band size: 36 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P07992

Gene ID

2067 [NCBI]

Immunogen

Synthetic Peptide of ERCC1

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	100-300
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200

Pureza	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4; Not functional in the nucleotide excision repair pathway; Not functional in the nucleotide excision repair pathway; Not functional in the nucleotide excision repair pathway

Subcellular Localization：Nucleus; Cytoplasm; Nucleus; Nucleus; Nucleus

Isoforms & Post-Translational Modification：P07992 has 4 isomers: P07992-1: 32562 Da (predicted); P07992-2: 29993 Da (predicted); P07992-3: 35563 Da (predicted); P07992-4: 25211 Da (predicted).
Ubiquitinated with both 'Lys-48' and 'Lys-63' linkages (PubMed:25538220). Deubiquitinated by USP45 (PubMed:25538220)

Subunit：Heterodimer composed of ERCC1 isoform 1 and ERCC4/XPF (PubMed:16076955, PubMed:16338413, PubMed:24036546, PubMed:25538220, PubMed:32034146). Interacts with USP45 (PubMed:25538220)

Synonyms

ERCC1; DNA excision repair protein ERCC-1

Documentación

User Guide for Antibodies (1077 KB)