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Junctophilin-2 Antibody (YA6621)

(Synonyms: FLJ40969 antibody; JP-2 antibody; JP2 antibody; Jph2 antibody; JPH2_HUMAN antibody; Junctophilin 2 antibody; Junctophilin type 2 antibody; Junctophilin-2 antibody; OTTHUMP00000031651 antibody; OTTHUMP00000031652 antibody; )
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Based on 1 Customer Validation

Junctophilin-2 Antibody (YA6621) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Junctophilin-2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Junctophilin-2 Antibody (YA6621)
  • Junctophilin-2 Antibody (YA6621) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:1000

Product Details

Description

Junctophilin-2 Antibody (YA6621) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Junctophilin-2.

  • Host Mouse
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 74 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 74 kDa
Immunogen

Synthetic peptide within Human Junctophilin-2 aa 1-50 / 696.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719228

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Junctophilin-2 Antibody (YA6621)
    Western blot analysis of extracts from C6(lane 2(20ug) and C6(lane 3(40ug) using Junctophilin-2 Antibody (HY-P86928) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Junctophilin-2 is a Membrane-binding protein that provides a structural bridge between the plasma membrane and the sarcoplasmic reticulum and is required for normal excitation-contraction coupling in cardiomyocytes. Provides a structural foundation for functional cross-talk between the cell surface and intracellular Ca(2+) release channels by maintaining the 12-15 nm gap between the sarcolemma and the sarcoplasmic reticulum membranes in the cardiac dyads. Necessary for proper intracellular Ca(2+) signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release. Contributes to the construction of skeletal muscle triad junctions|Transcription repressor required to safeguard against the deleterious effects of cardiac stress. Generated following cleavage of the Junctophilin-2 chain by calpain in response to cardiac stress in cardiomyocytes. Following cleavage and release from the membrane, translocates to the nucleus, binds DNA and represses expression of genes implicated in cell growth and differentiation, hypertrophy, inflammation and fibrosis. Modifies the transcription profile and thereby attenuates pathological remodeling in response to cardiac stress. Probably acts by competing with MEF2 transcription factors and TATA-binding proteins[1].

  • Subcellular Localization

    Cell membrane,Sarcoplasmic reticulum membrane,Endoplasmic reticulum membrane,Nucleus

  • Expression


    Tissue_Specificity: Specifically expressed in skeletal muscle and heart

  • Isoforms & Post-Translational Modification

    Q9BR39 has two isomers: Q9BR39-1: 74222 Da (predicted); Q9BR39-2: 13951 Da (predicted).
    Phosphorylation on Ser-165, probably by PKC, affects RYR1-mediated calcium ion release, interaction with TRPC3, and skeletal muscle myotubule development丨Proteolytically cleaved by calpain in response to cardiac stress

  • Subunit

    Interacts with TRPC3 (PubMed:20095964)

  • SwissProt ID

    Q9BR39

  • Gene ID
    57158 [NCBI]
  • Synonyms

    FLJ40969 antibody; JP-2 antibody; JP2 antibody; Jph2 antibody; JPH2_HUMAN antibody; Junctophilin 2 antibody; Junctophilin type 2 antibody; Junctophilin-2 antibody; OTTHUMP00000031651 antibody; OTTHUMP00000031652 antibody;

References

[1]. Woo JS, et al. S165F mutation of junctophilin 2 affects Ca2+ signalling in skeletal muscle. Biochem J. 2010 Mar 15;427(1):125-34. [Content Brief]

Junctophilin-2 Antibody (YA6621) Related Classifications

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Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) and C6(lane 3(40ug) using Junctophilin-2 Antibody (HY-P86928) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Junctophilin-2 Antibody (YA6621) Product Image
    1/16
  • Experimental Validation Results for Junctophilin-2 Antibody (YA6621)
  • Junctophilin-2 Antibody (YA6621) Product Image
  • Experimental Validation Results for Junctophilin-2 Antibody (YA6621)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) and C6(lane 3(40ug) using Junctophilin-2 Antibody (HY-P86928) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Junctophilin-2 Antibody (YA6621)
  • Junctophilin-2 Antibody (YA6621) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6(lane 2(20ug) and C6(lane 3(40ug) using Junctophilin-2 Antibody (HY-P86928) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Junctophilin-2 Antibody (YA6621) Product Image WB