Junctophilin-2 Antibody (YA6621)

Cat. No.: HY-P86928: Ficha de datos COA
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Junctophilin-2 Antibody (YA6621) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Junctophilin-2.

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Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn

Descripciòn

Junctophilin-2 Antibody (YA6621) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Junctophilin-2.

Host

Mouse

Clonality

Recombinant,Monoclonal

Peso molecular

Predicted band size: 74 kDa;

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9BR39

Gene ID

57158 [NCBI]

Immunogen

Synthetic peptide within Human Junctophilin-2 aa 1-50 / 696.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from C6(lane 2(20ug) and C6(lane 3(40ug) using Junctophilin-2 Antibody (HY-P86928) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Membrane-binding protein that provides a structural bridge between the plasma membrane and the sarcoplasmic reticulum and is required for normal excitation-contraction coupling in cardiomyocytes (PubMed:20095964). Provides a structural foundation for functional cross-talk between the cell surface and intracellular Ca(2+) release channels by maintaining the 12-15 nm gap between the sarcolemma and the sarcoplasmic reticulum membranes in the cardiac dyads (By similarity). Necessary for proper intracellular Ca(2+) signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release (By similarity). Contributes to the construction of skeletal muscle triad junctions (By similarity)|Transcription repressor required to safeguard against the deleterious effects of cardiac stress. Generated following cleavage of the Junctophilin-2 chain by calpain in response to cardiac stress in cardiomyocytes. Following cleavage and release from the membrane, translocates to the nucleus, binds DNA and represses expression of genes implicated in cell growth and differentiation, hypertrophy, inflammation and fibrosis. Modifies the transcription profile and thereby attenuates pathological remodeling in response to cardiac stress. Probably acts by competing with MEF2 transcription factors and TATA-binding proteins

Subcellular Localization：Cell membrane,Sarcoplasmic reticulum membrane,Endoplasmic reticulum membrane,Nucleus

Expression：
Tissue_Specificity: Specifically expressed in skeletal muscle and heart

Isoforms & Post-Translational Modification：Q9BR39 has two isomers: Q9BR39-1: 74222 Da (predicted); Q9BR39-2: 13951 Da (predicted).
Phosphorylation on Ser-165, probably by PKC, affects RYR1-mediated calcium ion release, interaction with TRPC3, and skeletal muscle myotubule development丨Proteolytically cleaved by calpain in response to cardiac stress

Subunit：Interacts with TRPC3 (PubMed:20095964)

RRID

AB_3719228

Synonyms

FLJ40969 antibody; JP-2 antibody; JP2 antibody; Jph2 antibody; JPH2_HUMAN antibody; Junctophilin 2 antibody; Junctophilin type 2 antibody; Junctophilin-2 antibody; OTTHUMP00000031651 antibody; OTTHUMP00000031652 antibody;

Documentación

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Ficha de datos (231 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)