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Myc Tag Antibody(HRP) (YA866)

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Based on 1 Customer Validation

Myc Tag Antibody(HRP) (YA866) is a HRP-conjugated, mouse-derived IgG1 monoclonal antibody, targeting to Myc Tag.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ELISA, IHC-P, IP

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    HRP
  • Experimental Validation Results for Myc Tag Antibody(HRP) (YA866)
  • Myc Tag Antibody(HRP) (YA866) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:5000-1:10000 1:50-1:200 1:500 1:5000-1:20000

Product Details

Description

Myc Tag Antibody(HRP) (YA866) is a HRP-conjugated, mouse-derived IgG1 monoclonal antibody, targeting to Myc Tag.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Species independent
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Immunogen

Synthetic peptide conjugated to Keyhole Limpet Haemocyanin (KLH).

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

HRP

Modification

Unmodified

Isotype

IgG1

RRID

AB_3103147

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Myc Tag Antibody(HRP) (YA866)
    Western blot analysis was performed on extracts from Recombinant c-Myc-Tag protein-10ng (lane 1, 10 ng), Recombinant c-Myc-Tag protein-5ng (lane 2, 5 ng), Recombinant c-Myc-Tag protein-2ng (lane 3, 2 ng), Recombinant c-Myc-Tag protein-1ng (lane 4, 1 ng), Recombinant c-Myc-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant c-Myc-Tag protein-0.1ng (lane 6, 0.1 ng) using c-Myc Tag (9E10) Mouse mAb (HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

Background

  • Function

    Myc Tag is a The Myc tag is a short peptide tag derived from the c-Myc protein. It has no biological function itself, but it can be used for the detection, localization, purification, and interaction studies of target proteins.

  • Research Field

    Tags & Cell Markers

Myc Tag Antibody(HRP) (YA866) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Recombinant c-Myc-Tag protein-10ng (lane 1, 10 ng), Recombinant c-Myc-Tag protein-5ng (lane 2, 5 ng), Recombinant c-Myc-Tag protein-2ng (lane 3, 2 ng), Recombinant c-Myc-Tag protein-1ng (lane 4, 1 ng), Recombinant c-Myc-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant c-Myc-Tag protein-0.1ng (lane 6, 0.1 ng) using c-Myc Tag (9E10) Mouse mAb (HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

  • Myc Tag Antibody(HRP) (YA866) Product Image
    1/16
  • Experimental Validation Results for Myc Tag Antibody(HRP) (YA866)
  • Myc Tag Antibody(HRP) (YA866) Product Image
  • Experimental Validation Results for Myc Tag Antibody(HRP) (YA866)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Recombinant c-Myc-Tag protein-10ng (lane 1, 10 ng), Recombinant c-Myc-Tag protein-5ng (lane 2, 5 ng), Recombinant c-Myc-Tag protein-2ng (lane 3, 2 ng), Recombinant c-Myc-Tag protein-1ng (lane 4, 1 ng), Recombinant c-Myc-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant c-Myc-Tag protein-0.1ng (lane 6, 0.1 ng) using c-Myc Tag (9E10) Mouse mAb (HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.
  • Experimental Validation Results for Myc Tag Antibody(HRP) (YA866)
  • Myc Tag Antibody(HRP) (YA866) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Recombinant c-Myc-Tag protein-10ng (lane 1, 10 ng), Recombinant c-Myc-Tag protein-5ng (lane 2, 5 ng), Recombinant c-Myc-Tag protein-2ng (lane 3, 2 ng), Recombinant c-Myc-Tag protein-1ng (lane 4, 1 ng), Recombinant c-Myc-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant c-Myc-Tag protein-0.1ng (lane 6, 0.1 ng) using c-Myc Tag (9E10) Mouse mAb (HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

  • Myc Tag Antibody(HRP) (YA866) Product Image WB