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  3. Primary Antibodies
  4. Monoclonal Antibodies Tag Antibodies Conjugated Primary Antibodies
  5. Myc Tag Antibody(HRP) (YA866)

Myc Tag Antibody(HRP) (YA866)

Cat. No.: HY-P80232A
COA
SDS
User Guide for Antibodies Technical Support

Myc Tag Antibody(HRP) (YA866) is a HRP-conjugated, mouse-derived IgG1 monoclonal antibody, targeting to Myc Tag.

For research use only. We do not sell to patients.

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20 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Documentation

Description

Myc Tag Antibody(HRP) (YA866) is a HRP-conjugated, mouse-derived IgG1 monoclonal antibody, targeting to Myc Tag.
Host

Mouse
Clonality

Monoclonal
Molecular Weight
Predicted band size: 50 kDa;
Observed band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Species independent
Immunogen

Synthetic peptide conjugated to Keyhole Limpet Haemocyanin (KLH).
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:10000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:200
IP
IP: Immunoprecipitation
1:500
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:5000-1:20000
Sensitivity Endogenous Purity Affinity Purified
Conjugation HRP Modification Unmodified
Isotype IgG1  
Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from Recombinant c-Myc-Tag protein-10ng (lane 1, 10 ng), Recombinant c-Myc-Tag protein-5ng (lane 2, 5 ng), Recombinant c-Myc-Tag protein-2ng (lane 3, 2 ng), Recombinant c-Myc-Tag protein-1ng (lane 4, 1 ng), Recombinant c-Myc-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant c-Myc-Tag protein-0.1ng (lane 6, 0.1 ng) using c-Myc Tag (9E10) Mouse mAb (HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

Background
Function：The Myc tag is a short peptide tag derived from the c-Myc protein. It has no biological function itself, but it can be used for the detection, localization, purification, and interaction studies of target proteins.
RRID
Research Field

Tags & Cell Markers
Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Myc Tag Antibody(HRP) (YA866) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Myc Tag Antibody(HRP) (YA866)
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