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HA Tag Antibody (HRP) (YA876)

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Based on 1 Customer Validation

HA Tag Antibody (HRP) (YA876) is a HA-conjugated, mouse-derived IgG1 monoclonal antibody, targeting to HA Tag.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ELISA

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    HRP
  • Experimental Validation Results for HA Tag Antibody (HRP) (YA876)
  • HA Tag Antibody (HRP) (YA876) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:1000-1:10000 1:5000-1:20000

Product Details

Description

HA Tag Antibody (HRP) (YA876) is a HA-conjugated, mouse-derived IgG1 monoclonal antibody, targeting to HA Tag.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Species independent
  • Observed Molecular Weight
    Observed band size: 48 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 48 kDa
Immunogen

YPYDVPDYA conjugated to KLH.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

HRP

Modification

Unmodified

Isotype

IgG1

RRID

AB_3103148

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HA Tag Antibody (HRP) (YA876)
    Western blot analysis was performed on extracts from Recombinant HA-Tag protein-10ng (lane 1, 10 ng), Recombinant HA-Tag protein-5ng (lane 2, 5 ng), Recombinant HA-Tag protein-2ng (lane 3, 2 ng), Recombinant HA-Tag protein-1ng (lane 4, 1 ng), Recombinant HA-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant HA-Tag protein-0.1ng (lane 6, 0.1 ng) using HA Tag Mouse mAb (HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

Background

  • Function

    HA Tag is a HA Peptide (HA tag) is a nine amino acids peptide derived from the human influenza hemagglutinin (HA). HA Peptide is extensively used to isolate, purify, detect, and track the protein of interest in cell biology and biochemistry.

  • Research Field

    Tags & Cell Markers

HA Tag Antibody (HRP) (YA876) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Recombinant HA-Tag protein-10ng (lane 1, 10 ng), Recombinant HA-Tag protein-5ng (lane 2, 5 ng), Recombinant HA-Tag protein-2ng (lane 3, 2 ng), Recombinant HA-Tag protein-1ng (lane 4, 1 ng), Recombinant HA-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant HA-Tag protein-0.1ng (lane 6, 0.1 ng) using HA Tag Mouse mAb (HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

  • HA Tag Antibody (HRP) (YA876) Product Image
    1/16
  • Experimental Validation Results for HA Tag Antibody (HRP) (YA876)
  • HA Tag Antibody (HRP) (YA876) Product Image
  • Experimental Validation Results for HA Tag Antibody (HRP) (YA876)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Recombinant HA-Tag protein-10ng (lane 1, 10 ng), Recombinant HA-Tag protein-5ng (lane 2, 5 ng), Recombinant HA-Tag protein-2ng (lane 3, 2 ng), Recombinant HA-Tag protein-1ng (lane 4, 1 ng), Recombinant HA-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant HA-Tag protein-0.1ng (lane 6, 0.1 ng) using HA Tag Mouse mAb (HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.
  • Experimental Validation Results for HA Tag Antibody (HRP) (YA876)
  • HA Tag Antibody (HRP) (YA876) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Recombinant HA-Tag protein-10ng (lane 1, 10 ng), Recombinant HA-Tag protein-5ng (lane 2, 5 ng), Recombinant HA-Tag protein-2ng (lane 3, 2 ng), Recombinant HA-Tag protein-1ng (lane 4, 1 ng), Recombinant HA-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant HA-Tag protein-0.1ng (lane 6, 0.1 ng) using HA Tag Mouse mAb (HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

  • HA Tag Antibody (HRP) (YA876) Product Image WB