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Flag Tag Antibody (HRP) (YA873)

Customer Review

Based on 1 Customer Validation

Flag Tag Antibody (HRP) (YA873) is a HRP-conjugated, mouse-derived IgG1 monoclonal antibody, targeting to Flag Tag.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    HRP
  • Experimental Validation Results for Flag Tag Antibody (HRP) (YA873)
  • Experimental Validation Results for Flag Tag Antibody (HRP) (YA873)
  • Flag Tag Antibody (HRP) (YA873) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-1:10000

Product Details

Description

Flag Tag Antibody (HRP) (YA873) is a HRP-conjugated, mouse-derived IgG1 monoclonal antibody, targeting to Flag Tag.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Species independent
Immunogen

DYKDDDDK synthetic peptide conjugated to KLH.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

HRP

Modification

Unmodified

Isotype

IgG1

RRID

AB_3103146

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Flag Tag Antibody (HRP) (YA873)
    Western blot analysis of protein Human SIRT5(lane 2(10ng) and lane 3(20ng)) and Human BRD4(lane 4(10ng) and lane 5(20ng)) using Flag Tag(HRP) (HY-P80111A) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) in 5% non-fat milk in TBST at 4°C overnight.
  • Experimental Validation Results for Flag Tag Antibody (HRP) (YA873)
    Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using Flag Tag Mouse mAb (HRP).Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

Background

  • Function

    Flag Tag is a FLAG Tag is a multifunctional fusion tag for the purification of recombinant proteins. FLAG Tag maintances the natural folding of its fusing proteins. FLAG Tag can be removed by enterokinase, and eluted under non-denaturing conditions.

  • Research Field

    Tags & Cell Markers

Flag Tag Antibody (HRP) (YA873) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of protein Human SIRT5(lane 2(10ng) and lane 3(20ng)) and Human BRD4(lane 4(10ng) and lane 5(20ng)) using Flag Tag(HRP) (HY-P80111A) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) in 5% non-fat milk in TBST at 4°C overnight.

  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using Flag Tag Mouse mAb (HRP).Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

  • Flag Tag Antibody (HRP) (YA873) Product Image
    1/16
  • Experimental Validation Results for Flag Tag Antibody (HRP) (YA873)
  • Experimental Validation Results for Flag Tag Antibody (HRP) (YA873)
  • Flag Tag Antibody (HRP) (YA873) Product Image
  • Experimental Validation Results for Flag Tag Antibody (HRP) (YA873)
    1/1
    MedchemExpress Validation
     Western blot analysis of protein Human SIRT5(lane 2(10ng) and lane 3(20ng)) and Human BRD4(lane 4(10ng) and lane 5(20ng)) using Flag Tag(HRP) (HY-P80111A) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) in 5% non-fat milk in TBST at 4°C overnight.
  • Experimental Validation Results for Flag Tag Antibody (HRP) (YA873)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using Flag Tag Mouse mAb (HRP).Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.
  • Experimental Validation Results for Flag Tag Antibody (HRP) (YA873)
  • Experimental Validation Results for Flag Tag Antibody (HRP) (YA873)
  • Flag Tag Antibody (HRP) (YA873) Product Image
    MedchemExpress Validation

    Western blot analysis of protein Human SIRT5(lane 2(10ng) and lane 3(20ng)) and Human BRD4(lane 4(10ng) and lane 5(20ng)) using Flag Tag(HRP) (HY-P80111A) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) in 5% non-fat milk in TBST at 4°C overnight.

  • Flag Tag Antibody (HRP) (YA873) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using Flag Tag Mouse mAb (HRP).Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

  • Flag Tag Antibody (HRP) (YA873) Product Image WB
  • Flag Tag Antibody (HRP) (YA873) Product Image WB