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  5. HRP-conjugated Goat Anti-Mouse IgG H&L

HRP-conjugated Goat Anti-Mouse IgG H&L

Cat. No.: HY-P8004
COA
SDS
User Guide for Antibodies Technical Support

HRP-conjugated Goat Anti-Mouse IgG H&L is a HRP-conjugated and Goat origined monoclonal antibody, targeting to Mouse IgG antibody. HRP-conjugated Goat Anti-Mouse IgG H&L can binds to the light and heavy chains of Mouse IgG antibodies.

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Top Publications Citing Use of Products

    HRP-conjugated Goat Anti-Mouse IgG H&L purchased from MCE. Usage Cited in: Parasit Vectors. 2025 Jun 20;18(1):230.  [Abstract]

    HRP-conjugated AffiniPure Goat Anti-Mouse IgG H&L. Representative images of IHC for MUC2, occludin, and ZO-1; percentages of MUC2, occludin, and ZO-1-positive areas were semi-quantified using Image.

    HRP-conjugated Goat Anti-Mouse IgG H&L purchased from MCE. Usage Cited in: Parasit Vectors. 2025 Jun 20;18(1):230.  [Abstract]

    HRP-conjugated AffiniPure Goat Anti-Mouse IgG H&L. WB results for occludin and ZO-1.

    HRP-conjugated Goat Anti-Mouse IgG H&L purchased from MCE. Usage Cited in: Parasit Vectors. 2025 Jun 20;18(1):230.  [Abstract]

    HRP-conjugated AffiniPure Goat Anti-Mouse IgG H&L. Representative images of IF for PCNA (green) and Lgr5 (green) and the nucleus (4′,6-diamidino-2-phenylindole (DAPI), blue) (scale bars 100 μm). Percentages of PCNA-positive area.
    • WB: Western Blot;
    • IHC-P: Immunohistochemistry-Paraffin;
    • IHC-F: Immunohistochemistry-Frozen;
    • ICC/IF: Immunocytochemistry/Immunofluorescence;
    • IF-Tissue: Immunofluorescence-Tissue;
    • mIHC: Multiplex Immunohistochemical;
    • IP: Immunoprecipitation;
    • ChIP: Chromatin Immunoprecipitation;
    • FC: Flow Cytometry;
    • ELISA: Enzyme Linked Immunosorbent Assay

    • Product Detail

    • Verification Image

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    • Description

    Description

    HRP-conjugated Goat Anti-Mouse IgG H&L is a HRP-conjugated and Goat origined monoclonal antibody, targeting to Mouse IgG antibody. HRP-conjugated Goat Anti-Mouse IgG H&L can binds to the light and heavy chains of Mouse IgG antibodies.
    Host

    Goat
    Clonality

    Polyclonal
    Species Reactivity
    Mouse
    Immunogen

    Synthetic peptide corresponding to Mouse IgG.
    Application &
    Dilution Ratio
    Application Dilution Ratio
    WB
    WB: Western Blot
    		1:5000-1:200000
    ELISA
    ELISA: Enzyme Linked Immunosorbent Assay
    		1:5000-1:100000
    IHC-P
    IHC-P: Immunohistochemistry-Paraffin
    		1:500-1:5000
    Sensitivity Endogenous Conjugation HRP
    Modification Unmodified  
    Appearance

    Solution
    Formulation

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
    Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
    Livraison

    Shipping with blue ice.
    Verification Image
    WB

    • Western blot analysis of extracts from Hela(lane 2(20μg), HepG2(lane 3(20μg), Jurkat(lane 4(20μg) ) and NIH3T3(lane 5(20μg) ) using HSP60 (HY-P80184) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80184, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

    RRID
    Documentation
    SDS (252 KB)

    COA (204 KB)

    User Guide for Antibodies (1077 KB)

    HRP-conjugated Goat Anti-Mouse IgG H&L Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    HRP-conjugated Goat Anti-Mouse IgG H&L
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    HY-P8004
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