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His Tag Antibody (HRP) (YA877)

1 Cited Publications
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Based on 1 publication(s) in Google Scholar

His Tag Antibody (HRP) (YA877) is a HRP-conjugated, mouse-derived IgG monoclonal antibody, targeting to His Tag.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    HRP
  • Experimental Validation Results for His Tag Antibody (HRP) (YA877)
  • Experimental Validation Results for His Tag Antibody (HRP) (YA877)
  • Experimental Validation Results for His Tag Antibody (HRP) (YA877)
  • His Tag Antibody (HRP) (YA877) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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1 Publications Citing Use of MCE His Tag Antibody (HRP) (YA877) (1)
WB
  • WB

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-1:10000

Product Details

Description

His Tag Antibody (HRP) (YA877) is a HRP-conjugated, mouse-derived IgG monoclonal antibody, targeting to His Tag.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Species independent
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 1 kDa
Immunogen

HHHHHH

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

HRP

Modification

Unmodified

Isotype

IgG

RRID

AB_3103030

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for His Tag Antibody (HRP) (YA877)
    Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using His-tag (HRP) (HY-P80947A) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for His Tag Antibody (HRP) (YA877)
    Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using His Tag Mouse mAb (HRP).Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

Background

  • Function

    His Tag is a His tags are short peptide sequences (usually composed of 6-10 consecutive histidine residues) fused to the N-terminus or C-terminus of a target protein through genetic engineering. They are used for the efficient and rapid purification of recombinant proteins using metal chelate affinity chromatography.

  • Research Field

    Tags & Cell Markers

His Tag Antibody (HRP) (YA877) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using His-tag (HRP) (HY-P80947A) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using His Tag Mouse mAb (HRP).Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

  • 1/16

    His Tag Antibody (HRP) (YA877) purchased from MedChemExpress. Usage Cited in: SSRN. 2025 Nov 20.

    Customer Validation
     Western blot analysis of proteins of interest expressed in ABMΔECN bacteria. Anti-His tag antibody (1:10000, His Tag Antibody (HRP) (YA877), HY-P80947A) detected Trx-A9R; anti-Flag tag antibody detected ClyA-B6R-M1R. Lanes: uninduced ΔEcN; ΔEcN + IPTG; uninduced BMΔEcN; BMΔEcN + IPTG; uninduced ABMΔEcN; ABMΔEcN + IPTG.

  • His Tag Antibody (HRP) (YA877) Product Image
    1/16
  • Experimental Validation Results for His Tag Antibody (HRP) (YA877)
  • Experimental Validation Results for His Tag Antibody (HRP) (YA877)
  • Experimental Validation Results for His Tag Antibody (HRP) (YA877)
  • His Tag Antibody (HRP) (YA877) Product Image
  • Experimental Validation Results for His Tag Antibody (HRP) (YA877)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using His-tag (HRP) (HY-P80947A) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for His Tag Antibody (HRP) (YA877)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using His Tag Mouse mAb (HRP).Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.
  • Experimental Validation Results for His Tag Antibody (HRP) (YA877)
  • Experimental Validation Results for His Tag Antibody (HRP) (YA877)

  • His Tag Antibody (HRP) (YA877) purchased from MedChemExpress. Usage Cited in: SSRN. 2025 Nov 20.

    Western blot analysis of proteins of interest expressed in ABMΔECN bacteria. Anti-His tag antibody (1:10000, His Tag Antibody (HRP) (YA877), HY-P80947A) detected Trx-A9R; anti-Flag tag antibody detected ClyA-B6R-M1R. Lanes: uninduced ΔEcN; ΔEcN + IPTG; uninduced BMΔEcN; BMΔEcN + IPTG; uninduced ABMΔEcN; ABMΔEcN + IPTG.

  • His Tag Antibody (HRP) (YA877) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using His-tag (HRP) (HY-P80947A) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • His Tag Antibody (HRP) (YA877) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using His Tag Mouse mAb (HRP).Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

  • His Tag Antibody (HRP) (YA877) Product Image WB
  • His Tag Antibody (HRP) (YA877) Product Image WB