His Tag Antibody (HRP) (YA877)

製品番号: HY-P80947A: データシート SDS COA User Guide for Antibodies
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His Tag Antibody (HRP) (YA877) is a HRP-conjugated, mouse-derived IgG monoclonal antibody, targeting to His Tag.

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容量	価格（税別）	在庫状況	数量
20 μL	$108	在庫あり	Estimated Time of Arrival: December 31
50 μL	$155	在庫あり	Estimated Time of Arrival: December 31
100 μL	$245	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

顧客検証

•SSRN. 2025 Nov 20.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

His Tag Antibody (HRP) (YA877) is a HRP-conjugated, mouse-derived IgG monoclonal antibody, targeting to His Tag.

Host

Mouse

Clonality

Monoclonal

分子量

Predicted band size: 1 kDa;

Observed band size: 47 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Species independent

Immunogen

HHHHHH

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:10000

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	HRP	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using His-tag (HRP) (HY-P80947A) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis was performed on extracts from K005-10ng (lane 1, 10ng), K005-5ng (lane 2, 5ng), K005-2ng (lane 3, 2ng), K005-1ng (lane 4, 1ng), K005-0.5ng (lane 5, 0.5ng), and K005-0.1ng (lane 6, 0.1ng) using His Tag Mouse mAb (HRP).Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

Background

Function：His tags are short peptide sequences (usually composed of 6-10 consecutive histidine residues) fused to the N-terminus or C-terminus of a target protein through genetic engineering. They are used for the efficient and rapid purification of recombinant proteins using metal chelate affinity chromatography.

RRID

AB_3103030

Research Field

Tags & Cell Markers

ドキュメンテーション

Select Batch:

データシート (230 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)