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NPR3 Antibody (YA1541)

(Synonyms: ANPC; ANPRC; GUCY2B; NPR 3; NPR C; Npr3; NPRC)
Customer Review

Based on 1 Customer Validation

NPR3 Antibody (YA1541) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NPR3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NPR3 Antibody (YA1541)
  • NPR3 Antibody (YA1541) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:50-1:100

Product Details

Description

NPR3 Antibody (YA1541) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NPR3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size:90 kD Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Natriuretic Peptide Receptor C aa500-541/541.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NPR3 Antibody (YA1541)
    Western blot analysis was performed on protein extracts (30 μg) from Mouse kidney (lane 2), Rat kidney (lane 3), Raji (lane 4), SH-SY5Y (lane 5), and A549 (lane 6) using NPR3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    NPR3 receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. Acts as a regulator of osteoblast differentiation and bone growth by binding to its ligand osteocrin, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Isoforms & Post-Translational Modification

    P17342 has 3 isomers: P17342-1: 59808 Da (predicted); P17342-2: 59767 Da (predicted); P17342-3: 36656 Da (predicted).

  • Subunit

    Homodimer; disulfide-linked (PubMed:11533490). Dimers can also be formed through the C-terminal cysteine of isoform 2 (PubMed:11533490). Interacts with OSTN (By similarity)

  • SwissProt ID

    P17342

  • Gene ID
    4883 [NCBI]
  • Synonyms

    ANPC; ANPRC; GUCY2B; NPR 3; NPR C; Npr3; NPRC

  • Research Field

    Signal Transduction

NPR3 Antibody (YA1541) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Mouse kidney (lane 2), Rat kidney (lane 3), Raji (lane 4), SH-SY5Y (lane 5), and A549 (lane 6) using NPR3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • NPR3 Antibody (YA1541) Product Image
    1/16
  • Experimental Validation Results for NPR3 Antibody (YA1541)
  • NPR3 Antibody (YA1541) Product Image
  • Experimental Validation Results for NPR3 Antibody (YA1541)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Mouse kidney (lane 2), Rat kidney (lane 3), Raji (lane 4), SH-SY5Y (lane 5), and A549 (lane 6) using NPR3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for NPR3 Antibody (YA1541)
  • NPR3 Antibody (YA1541) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from Mouse kidney (lane 2), Rat kidney (lane 3), Raji (lane 4), SH-SY5Y (lane 5), and A549 (lane 6) using NPR3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • NPR3 Antibody (YA1541) Product Image WB