LIMA1 Antibody (YA8156)

Cat. No.: HY-P88472: Data Sheet User Guide for Antibodies
FAQs
Technical Support

LIMA1 Antibody (YA8156) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LIMA1.

For research use only. We do not sell to patients.

Size	Price	Stock
20 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

LIMA1 Antibody (YA8156) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LIMA1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 85 kDa

Species Reactivity

Human

SwissProt ID

Q9UHB6

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-275 of human LIMA1 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Solution

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Shipping

Shipping with blue ice.

Background

Function：Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (PubMed:12566430, PubMed:33999101). Acts as a negative regulator of primary cilium formation (PubMed:32496561). Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (By similarity)

Subcellular Localization：Cytoplasm,Cell junction, focal adhesion,Cytoplasm, cytoskeleton,Cytoplasm, cytoskeleton, stress fiber,Cell membrane,Cell projection, ruffle,Cell projection, lamellipodium

Expression：
Tissue_Specificity: Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium
Induction: Down-regulated in some cancer cell lines. Isoform Alpha is induced by serum. Isoform Beta is constitutively expressed

Isoforms & Post-Translational Modification：Q9UHB6 has 5 isomers: Q9UHB6-1: 85226 Da (predicted); Q9UHB6-2: 67119 Da (predicted); Q9UHB6-3: 51219 Da (predicted); Q9UHB6-4: 85224 Da (predicted); Q9UHB6-5: 67117 Da (predicted).
Ubiquitinated by the ECS(RAB40B) complex leading to its degradation丨Phosphorylation of the C-terminal region by MAPK1/MAPK3 reduces its association with F-actin and contributes to actin filament reorganization and enhances cell motility

Subunit：Interacts with NPC1L1; bridges NPC1L1 with MYO5B (PubMed:29880681)

Synonyms

EPLIN; SREBP3

Documentation

User Guide for Antibodies (1077 KB)