Copy Link Print Information

STAT5a+b Antibody (YA054)

(Synonyms: MGF; STAT5)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

STAT5a+b Antibody (YA054) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STAT5a+b.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for STAT5a+b Antibody (YA054)
  • Experimental Validation Results for STAT5a+b Antibody (YA054)
  • STAT5a+b Antibody (YA054) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
You May Still Need
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) STAT5a+b Antibody (YA054)

More

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

STAT5a+b Antibody (YA054) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STAT5a+b.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 91 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 91 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Mouse STAT5A aa600-786.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102249

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for STAT5a+b Antibody (YA054)
    Western blot analysis was performed on extracts from PC-12 (lane 1, 20 μg), C6 (lane 2, 20 μg), RAW264.7 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), HeLa (lane 5, 20 μg), and A549 (lane 6, 20 μg) using STAT5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for STAT5a+b Antibody (YA054)
    Western blot analysis of extracts from 293T (lane 1) and K562 (lane 2) and HepG2 (lane 3) using STAT5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:In adolescent females, it is present in almost all tissues except the brain and muscles. In lactating women, it is abundant in mammary gland tissue and other secretory organs (such as salivary glands and seminal vesicles) .

  • Subunit

    Forms a homodimer or a heterodimer with a related family member. Interacts with NCOA1 and SOCS7 (By similarity). Binds NR3C1 (PubMed:9528750). Interacts with ERBB4 (PubMed:10508857, PubMed:16837552). Interacts with EBF4 (By similarity). Interacts with CD69 (PubMed:20696842)

  • SwissProt ID

    P42230

  • Gene ID
    6776 [NCBI]
  • Synonyms

    MGF; STAT5

  • Research Field

    Signal Transduction

STAT5a+b Antibody (YA054) Related Classifications

100 mg

Get Quote In-stock

Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    STAT5a+b Antibody (YA054) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Western blot analysis was performed on extracts from PC-12 (lane 1, 20 μg), C6 (lane 2, 20 μg), RAW264.7 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), HeLa (lane 5, 20 μg), and A549 (lane 6, 20 μg) using STAT5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    STAT5a+b Antibody (YA054) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Western blot analysis of extracts from 293T (lane 1) and K562 (lane 2) and HepG2 (lane 3) using STAT5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • STAT5a+b Antibody (YA054) Product Image
    1/16
  • Experimental Validation Results for STAT5a+b Antibody (YA054)
  • Experimental Validation Results for STAT5a+b Antibody (YA054)
  • STAT5a+b Antibody (YA054) Product Image
  • Experimental Validation Results for STAT5a+b Antibody (YA054)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from PC-12 (lane 1, 20 μg), C6 (lane 2, 20 μg), RAW264.7 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), HeLa (lane 5, 20 μg), and A549 (lane 6, 20 μg) using STAT5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for STAT5a+b Antibody (YA054)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from 293T (lane 1) and K562 (lane 2) and HepG2 (lane 3) using STAT5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for STAT5a+b Antibody (YA054)
  • Experimental Validation Results for STAT5a+b Antibody (YA054)
  • STAT5a+b Antibody (YA054) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from PC-12 (lane 1, 20 μg), C6 (lane 2, 20 μg), RAW264.7 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), HeLa (lane 5, 20 μg), and A549 (lane 6, 20 μg) using STAT5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • STAT5a+b Antibody (YA054) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from 293T (lane 1) and K562 (lane 2) and HepG2 (lane 3) using STAT5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • STAT5a+b Antibody (YA054) Product Image WB
  • STAT5a+b Antibody (YA054) Product Image WB