Phospho-STAT5a (Tyr694) Antibody (YA145)

Cat. No.: HY-P80479: Data Sheet COA
SDS
User Guide for Antibodies
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Phospho-STAT5a (Tyr694) Antibody (YA145) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-STAT5a (Tyr694).

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Phospho-STAT5a (Tyr694) Antibody (YA145) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Phospho-STAT5a (Tyr694) Antibody (YA145) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-STAT5a (Tyr694).

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 91 kDa;

Observed band size: 91 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat, Mouse

SwissProt ID

P42229

Gene ID

6776 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Tyr694 of Human STAT5A.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from K562 (lane 2(20μg), K562 (lane 3(40μg), using Phospho-STAT5 (Tyr694) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling Phospho-STAT5 (Tyr694) with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of HepG2 cells labeling Phospho-STAT5 (Tyr694) with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT5 (Tyr694) Antibody (HY-P80479)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation

Subcellular Localization：Cytoplasm; Nucleus

Subunit：Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7 (PubMed:12954634). Interacts with ERBB4 (PubMed:15534001). Interacts with EBF4 (PubMed:35939714). Interacts with CD69 (By similarity)

RRID

AB_3102552

Database

Entrez Gene: 6776 Human ; 6777 Human ; 20850 Mouse ; 20851 Mouse ; 24918 Rat 25126

SwissProt: P42229 Human ; P51692 Human ; P42230 Mouse ; P42232 Mouse ; P52632 Rat Q62771

OMIM: 601511 Human

Research Field

Signal Transduction

Synonyms

STAT5A; STAT5; Signal transducer and activator of transcription 5A

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-STAT5a (Tyr694) Antibody (YA145) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-STAT5a (Tyr694) Antibody (YA145)

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Background

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Phospho-STAT5a (Tyr694) Antibody (YA145) Related Classifications

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