Copy Link Print Information

Phospho-STAT5a (Tyr694) Antibody (YA145)

(Synonyms: STAT5A; STAT5; Signal transducer and activator of transcription 5A)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

Phospho-STAT5a (Tyr694) Antibody (YA145) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-STAT5a (Tyr694).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Rat, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
  • Phospho-STAT5a (Tyr694) Antibody (YA145) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Phospho-STAT5a (Tyr694) Antibody (YA145)

More

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:2000 1:50-1:100 1:100

Product Details

Description

Phospho-STAT5a (Tyr694) Antibody (YA145) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-STAT5a (Tyr694).

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Rat, Mouse
  • Observed Molecular Weight
    Observed band size: 91 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 91 kDa
Species Reactivity Database
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Tyr694 of Human STAT5A.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102552

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
    Western blot analysis of extracts from K562 (lane 2(20μg), K562 (lane 3(40μg), using Phospho-STAT5 (Tyr694) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
    Immunocytochemistry analysis of HepG2 cells labeling Phospho-STAT5 (Tyr694) with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
    Immunocytochemistry analysis of HepG2 cells labeling Phospho-STAT5 (Tyr694) with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT5 (Tyr694) Antibody (HY-P80479)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    STAT5a is a Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Subunit

    Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7 (PubMed:12954634). Interacts with ERBB4 (PubMed:15534001). Interacts with EBF4 (PubMed:35939714). Interacts with CD69 (By similarity)

  • SwissProt ID

    P42229

  • Gene ID
    6776 [NCBI]
  • Synonyms

    STAT5A; STAT5; Signal transducer and activator of transcription 5A

  • Research Field

    Signal Transduction

Phospho-STAT5a (Tyr694) Antibody (YA145) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), K562 (lane 3(40μg), using Phospho-STAT5 (Tyr694) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Phospho-STAT5 (Tyr694) with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Phospho-STAT5 (Tyr694) with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT5 (Tyr694) Antibody (HY-P80479)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-STAT5a (Tyr694) Antibody (YA145) Product Image
    1/16
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
  • Phospho-STAT5a (Tyr694) Antibody (YA145) Product Image
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), K562 (lane 3(40μg), using Phospho-STAT5 (Tyr694) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Phospho-STAT5 (Tyr694) with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Phospho-STAT5 (Tyr694) with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT5 (Tyr694) Antibody (HY-P80479)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
  • Experimental Validation Results for Phospho-STAT5a (Tyr694) Antibody (YA145)
  • Phospho-STAT5a (Tyr694) Antibody (YA145) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg), K562 (lane 3(40μg), using Phospho-STAT5 (Tyr694) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Phospho-STAT5a (Tyr694) Antibody (YA145) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Phospho-STAT5 (Tyr694) with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-STAT5a (Tyr694) Antibody (YA145) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Phospho-STAT5 (Tyr694) with Phospho-STAT5 (Tyr694) Antibody (HY-P80479) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT5 (Tyr694) Antibody (HY-P80479)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-STAT5a (Tyr694) Antibody (YA145) Product Image WB
  • Phospho-STAT5a (Tyr694) Antibody (YA145) Product Image ICC
  • Phospho-STAT5a (Tyr694) Antibody (YA145) Product Image ICC