RANKL Antibody

Art. -Nr.: HY-P81124: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

RANKL Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to RANKL.

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Größe	Verfügbarkeit	Menge
20 μL	Auf Lager	Estimated Time of Arrival: December 31
50 μL	Auf Lager	Estimated Time of Arrival: December 31
100 μL	Auf Lager	Estimated Time of Arrival: December 31
250 μL	Angebot einholen

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RANKL Antibody Spezialitätsempfehlungen:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Beschreibung

Beschreibung

RANKL Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to RANKL.

Host

Rabbit

Clonality

Polyclonal

Molekulargewicht

Predicted band size: 35 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O14788

Gene ID

8600 [NCBI]

Immunogen

The antiserum was produced against synthesized peptide derived from the C-terminal region of human TNFSF11.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Reinheit	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from 293(lane 2, 20μg), HeLa(lane 3, 20μg), Mouse Spleen(lane 4, 20μg) and Rat Spleen(lane 5, 20μg) using RANKL Antibody (HY-P81124). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

Background

Function：Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed:22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity)

Subcellular Localization：Cell membrane; Single-pass type II membrane protein; Cell membrane; Single-pass type II membrane protein; Cytoplasm; Secreted

Expression：
Tissue_specificity:It has the highest content in peripheral lymph nodes, and lower content in spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid gland.

Induction:Up-regulated by T-cell receptor stimulation

Isoforms & Post-Translational Modification：O14788 has 3 isomers: O14788-1: 35478 Da (predicted); O14788-2: 27690 Da (predicted); O14788-3: 30523 Da (predicted).
The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17

Subunit：Homotrimer (By similarity). Interacts with TNFRSF11B (PubMed:22664871). Interacts with TNFRSF11A. Interacts with FBN1 (via N-terminal domain) in a Ca(+2)-dependent manner (By similarity). Interacts with TNFAIP6 (via both Link and CUB domains)

RRID

AB_3103225

Database

Entrez Gene: 8600 Human ; 21943 Mouse ; 117516 Rat

SwissProt: O14788 Human ; O35235 Mouse ; Q9ESE2 Rat

OMIM: 259710 Human

Synonyms

OPGL; CD254; hRANKL2; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; RANKL; Receptor activator of nuclear factor kappa B ligand; sOdf; SOFA; TNF related activation induced cytokine; TNFSF 11; TNFSF11; TRANCE; Tumor necrosis factor ligand superfamily member 11; Osteoprotegerin Ligand; TNF11_HUMAN.

Dokumentation

Select Batch:

Data Sheet (258 KB) SDS (252 KB)

COA (204 KB)

User Guide for Antibodies (1077 KB)

RANKL Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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RANKL Antibody

RANKL Antibody Spezialitätsempfehlungen:

Product Detail

Verification Image

Background

Beschreibung

RANKL Antibody Related Classifications

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