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Cleaved-Caspase 9 Antibody (YA852)

(Synonyms: MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6)
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Based on 4 publication(s) in Google Scholar

Cleaved-Caspase 9 Antibody (YA852) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-Caspase 9.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Cleaved-Caspase 9 Antibody (YA852) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Cleaved-Caspase 9 Antibody (YA852) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-Caspase 9.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 46/39/37/35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human cleaved Caspase-9 aa1-200.

Sensitivity

Endogenous

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102010

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    Cleaved-Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC; Lacks activity is an dominant-negative inhibitor of caspase-9[1][2].

  • Expression


    Tissue_specificity:It is widely distributed throughout the body, with the highest expression level in the heart, moderate expression levels in the liver, skeletal muscle, and pancreas, and low expression levels in all other tissues. In the heart, it is specifically expressed in cardiomyocytes.

  • Isoforms & Post-Translational Modification

    P55211 has 4 isomers: P55211-1: 46281 Da (predicted); P55211-2: 30184 Da (predicted); P55211-3: 17397 Da (predicted); P55211-4: 36564 Da (predicted).
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events;Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage;(Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP9 processing, preventing CASP9 activation and ability to mediate intrinsic apoptosis;Ubiquitinated by BIRC6; this activity is inhibited by DIABLO/SMAC

  • Subunit

    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts (inactive form) with EFHD2. Interacts with HAX1. Interacts with BIRC2/c-IAP1, XIAP/BIRC4, BIRC5/survivin, BIRC6/bruce and BIRC7/livin. Interacts with ABL1 (via SH3 domain); the interaction is direct and increases in the response of cells to genotoxic stress and ABL1/c-Abl activation. Interacts with BCL2L10 (PubMed:19255499). Interacts with NleF from pathogenic E.coli

  • SwissProt ID

    P55211

  • Gene ID
    842 [NCBI]
  • Synonyms

    MCH6, CASP9, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, APAF-3, ICE-LAP6

[1]. Ehrmann JF, et al. Structural basis for regulation of apoptosis and autophagy by the BIRC6/SMAC complex. Science. 2023 Mar 17;379(6637):1117-1123. [Content Brief]

[2]. Dietz L, et al. Structural basis for SMAC-mediated antagonism of caspase inhibition by the giant ubiquitin ligase BIRC6. Science. 2023 Mar 17;379(6637):1112-1117. [Content Brief]

Cleaved-Caspase 9 Antibody (YA852) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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