Cleaved-Caspase 9 Antibody (YA852)

Cat. No.: HY-P80964: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
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Cleaved-Caspase 9 Antibody (YA852) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-Caspase 9.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Cleaved-Caspase 9 Antibody (YA852) Featured Recommendations:

4 Publications Citing Use of MCE Cleaved-Caspase 9 Antibody (YA852)

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Cleaved-Caspase 9 Antibody (YA852) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-Caspase 9.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 46 kDa;

Observed band size: 46/39/37/35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P55211

Gene ID

842 [NCBI]

Immunogen

A synthesized peptide derived from human cleaved Caspase-9 aa1-200.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758105, PubMed:36758106); Lacks activity is an dominant-negative inhibitor of caspase-9

Expression：
Tissue_specificity:It is widely distributed throughout the body, with the highest expression level in the heart, moderate expression levels in the liver, skeletal muscle, and pancreas, and low expression levels in all other tissues. In the heart, it is specifically expressed in cardiomyocytes.

Isoforms & Post-Translational Modification：P55211 has 4 isomers: P55211-1: 46281 Da (predicted); P55211-2: 30184 Da (predicted); P55211-3: 17397 Da (predicted); P55211-4: 36564 Da (predicted).
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events;Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage;(Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP9 processing, preventing CASP9 activation and ability to mediate intrinsic apoptosis;Ubiquitinated by BIRC6; this activity is inhibited by DIABLO/SMAC

Subunit：Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts (inactive form) with EFHD2. Interacts with HAX1. Interacts with BIRC2/c-IAP1, XIAP/BIRC4, BIRC5/survivin, BIRC6/bruce and BIRC7/livin. Interacts with ABL1 (via SH3 domain); the interaction is direct and increases in the response of cells to genotoxic stress and ABL1/c-Abl activation. Interacts with BCL2L10 (PubMed:19255499). Interacts with NleF from pathogenic E.coli

RRID

AB_3102010

Database

Entrez Gene: 842 Human ; 12371 Mouse ;

SwissProt: P55211 Human ; Q8C3Q9 Mouse ;

OMIM: 602234 Human

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Cleaved-Caspase 9 Antibody (YA852) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cleaved-Caspase 9 Antibody (YA852)

Cleaved-Caspase 9 Antibody (YA852) Featured Recommendations:

4 Publications Citing Use of MCE Cleaved-Caspase 9 Antibody (YA852)

Product Detail

Background

Documentation

Cleaved-Caspase 9 Antibody (YA852) Related Classifications

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